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Literature summary for 5.3.4.1 extracted from

  • Kimura, S.; Higashino, Y.; Kitao, Y.; Masuda, T.; Urade, R.
    Expression and characterization of protein disulfide isomerase family proteins in bread wheat (2015), BMC Plant Biol., 15, 73 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.4.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Triticum aestivum

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Triticum aestivum 5783
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
reduced ribonuclease A Triticum aestivum
-
 denatured ribonuclease A
-
 ?

Organism

Organism UniProt Comment Textmining
Triticum aestivum A0A024FRA5
-
-
Triticum aestivum A0A024FRN3
-
-
Triticum aestivum A0A024FRN8
-
-
Triticum aestivum D8L9A3
-
-
Triticum aestivum D8L9B3
-
-
Triticum aestivum Q7FYS2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose 6 column chromatography, and TSK gel G3000SW gel filtration Triticum aestivum

Source Tissue

Source Tissue Comment Organism Textmining
aleurone layer
-
 Triticum aestivum
-
endosperm
-
 Triticum aestivum
-
seed
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.175
-
 isoform PDIL5A, at pH 7.5 and 25°C Triticum aestivum
0.179
-
 isoform PDIL4D, at pH 7.5 and 25°C Triticum aestivum
0.287
-
 isoform PDIL2, at pH 7.5 and 25°C Triticum aestivum
0.665
-
 isoform PDIL1B, at pH 7.5 and 25°C Triticum aestivum
0.736
-
 isoform PDIL1Aalpha, at pH 7.5 and 25°C Triticum aestivum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
reduced ribonuclease A
-
 Triticum aestivum denatured ribonuclease A
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, isoform PDIL4D, SDS-PAGE Triticum aestivum
? x * 47000, isoform PDIL5A, SDS-PAGE Triticum aestivum
? x * 60000, isoform PDIL1Aalpha, SDS-PAGE Triticum aestivum
? x * 60000, isoform PDIL1B, SDS-PAGE Triticum aestivum
? x * 69000, isoform PDIL3A, SDS-PAGE Triticum aestivum
? x * 70000, isoform PDIL2, SDS-PAGE Triticum aestivum

Synonyms

Synonyms Comment Organism
PDIL1Aalpha isoform Triticum aestivum
PDIL1B isoform Triticum aestivum
PDIL2 isoform Triticum aestivum
PDIL3A isoform Triticum aestivum
PDIL4D isoform Triticum aestivum
PDIL5A isoform Triticum aestivum
protein disulfide isomerase
-
 Triticum aestivum