Application | Comment | Organism |
---|---|---|
medicine | PDI represents a potential drug target for anti-amebic therapy | Entamoeba histolytica |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli XL1-Blue MRF' cells | Entamoeba histolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bacitracin | - |
Entamoeba histolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0116 | - |
scrambled reoxidized lysozyme | pH 7.0, temperature not specified in the publication | Entamoeba histolytica | |
0.0253 | - |
denatured-reduced lysozyme | pH 7.0, temperature not specified in the publication | Entamoeba histolytica | |
2.026 | - |
Insulin | pH 7.0, temperature not specified in the publication | Entamoeba histolytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA-agarose column chromatography, gel filtration | Entamoeba histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
denatured-reduced lysozyme | oxidase activity of PDI | Entamoeba histolytica | ? | - |
? | |
insulin | reductase activity of PDI | Entamoeba histolytica | ? | - |
? | |
additional information | PDI behaves mainly as an oxidase/isomerase and exhibits chaperone-like activity | Entamoeba histolytica | ? | - |
? | |
RNase A | oxidase activity of PDI | Entamoeba histolytica | ? | - |
? | |
scrambled reoxidized lysozyme | isomerase activity of PDI | Entamoeba histolytica | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PDI | - |
Entamoeba histolytica |
protein disulfide isomerase | - |
Entamoeba histolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
pH optimum for the reductase activity of recombinant PDI | Entamoeba histolytica |
8 | - |
pH optimum for the oxidase activity of recombinant PDI | Entamoeba histolytica |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.172 | - |
using denatured-reduced lysozyme as substrate, at pH 7.0, temperature not specified in the publication | Entamoeba histolytica | bacitracin | |
0.334 | - |
using scrambled reoxidized lysozyme as substrate, at pH 7.0, temperature not specified in the publication | Entamoeba histolytica | bacitracin | |
1.137 | - |
using insulin as substrate, at pH 7.0, temperature not specified in the publication | Entamoeba histolytica | bacitracin |