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Literature summary for 5.3.4.1 extracted from

  • Kozlov, G.; Maattanen, P.; Schrag, J.D.; Pollock, S.; Cygler, M.; Nagar, B.; Thomas, D.Y.; Gehring, K.
    Crystal structure of the bb domains of the protein disulfide isomerase ERp57 (2006), Structure, 14, 1331-1339.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
calnexin interacts with the enzyme via the b and b' domains, binding site and structure, overview Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of GST-fusion full-length ERp57 lacking the signal sequence, A24-L505, and the bb', P134-G376, and b', F241-G376, fragments in Escherichia coli strain BL21(DE3) Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
ERp57 and its isolated bb' and b' domains in complex with the lectin chaperone calnexin, hanging drop vapour diffusion method, equilibration of 14 mg/ml protein in 50 mM Tris-HCl, 0.15 M NaCl, 1 mM DTT, pH 7.5, against 30% w/v PEG 3350, 0.1 M (NH4)2SO4, 0.1 M HEPES buffer, pH 7.5, for 1-3 days at 20°C, X-ray diffraction structure determination and analysis at 2.0 A resoution Homo sapiens

Protein Variants

Protein Variants Comment Organism
K274A site-directed mutagenesis, the mutant shows affected interaction with calnexin Homo sapiens
K332A site-directed mutagenesis, the mutant shows binding affinity with calnexin identical to wild-type enzyme Homo sapiens
R282A site-directed mutagenesis, the mutant shows affected interaction with calnexin Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
Homo sapiens 5783
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens PDIis responsible for correct disulfide bond formation of proteins in the endoplasmic reticulum, it recognize unfolded proteins and can be selective for specific proteins or classes ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P30101
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information PDIis responsible for correct disulfide bond formation of proteins in the endoplasmic reticulum, it recognize unfolded proteins and can be selective for specific proteins or classes Homo sapiens ?
-
?
additional information ERp57 interacts with the lectin chaperone calnexin, binding structure, and active site structure, overview Homo sapiens ?
-
?
RNase B the ability of the ERp57-calnexin complex to mediate folding of 3H-labeled RNase B is completely dependent on a functional interaction between ERp57 and calnexin, overview Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
More determination and analysis of the crystal structure of the bb domains of ERp57, overview Homo sapiens

Synonyms

Synonyms Comment Organism
ERp57
-
Homo sapiens
More the enzyme belongs to the protein disulfide isomerase family Homo sapiens
PDI
-
Homo sapiens
protein disulfide isomerase
-
Homo sapiens