Activating Compound | Comment | Organism | Structure |
---|---|---|---|
calnexin | interacts with the enzyme via the b and b' domains, binding site and structure, overview | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of GST-fusion full-length ERp57 lacking the signal sequence, A24-L505, and the bb', P134-G376, and b', F241-G376, fragments in Escherichia coli strain BL21(DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
ERp57 and its isolated bb' and b' domains in complex with the lectin chaperone calnexin, hanging drop vapour diffusion method, equilibration of 14 mg/ml protein in 50 mM Tris-HCl, 0.15 M NaCl, 1 mM DTT, pH 7.5, against 30% w/v PEG 3350, 0.1 M (NH4)2SO4, 0.1 M HEPES buffer, pH 7.5, for 1-3 days at 20°C, X-ray diffraction structure determination and analysis at 2.0 A resoution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K274A | site-directed mutagenesis, the mutant shows affected interaction with calnexin | Homo sapiens |
K332A | site-directed mutagenesis, the mutant shows binding affinity with calnexin identical to wild-type enzyme | Homo sapiens |
R282A | site-directed mutagenesis, the mutant shows affected interaction with calnexin | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | PDIis responsible for correct disulfide bond formation of proteins in the endoplasmic reticulum, it recognize unfolded proteins and can be selective for specific proteins or classes | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P30101 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | PDIis responsible for correct disulfide bond formation of proteins in the endoplasmic reticulum, it recognize unfolded proteins and can be selective for specific proteins or classes | Homo sapiens | ? | - |
? | |
additional information | ERp57 interacts with the lectin chaperone calnexin, binding structure, and active site structure, overview | Homo sapiens | ? | - |
? | |
RNase B | the ability of the ERp57-calnexin complex to mediate folding of 3H-labeled RNase B is completely dependent on a functional interaction between ERp57 and calnexin, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | determination and analysis of the crystal structure of the bb domains of ERp57, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ERp57 | - |
Homo sapiens |
More | the enzyme belongs to the protein disulfide isomerase family | Homo sapiens |
PDI | - |
Homo sapiens |
protein disulfide isomerase | - |
Homo sapiens |