Cloned (Comment) | Organism |
---|---|
genes dsbA and dsbC, DNA sequence determination and analysis, expression of His-tagged DsbA and DsbC in Escherichia coli strain BL(DE3) | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
C34S | site-directed mutagenesis, the mutant shows impaired lipase activity, the defect cannot be rescued by constitutive expression of lipA gene encoding lipase A | Pseudomonas aeruginosa |
additional information | construction of mutants deficient in either DsbA or DsbC, or both, the dsbA mutant does no longer produce extracellular enzymes elastase, alkaline phosphatase, and lipase, while in the dcbC mutant the lipase activity is doubled, introduction of dsbC into the dsbA mutant results in low extracellular lipase activity, overview | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Pseudomonas aeruginosa | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
2 * 22000, DsbA, SDS-PAGE, 2 * 25000, DsbC, SDS-PAGE | Pseudomonas aeruginosa |
25000 | - |
2 * 22000, DsbA, SDS-PAGE, 2 * 25000, DsbC, SDS-PAGE | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas aeruginosa | DsbA and DsbC are involved in disulfide bond formation and play an important role in the formation of extracellular enzymes, DsbA is important in lipase stability and excretion | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
genes dsbA and dsbC | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged DsbA and DsbC from Escherichia coli strain BL(DE3) by nickel affinity chromatography | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | DsbA and DsbC are involved in disulfide bond formation and play an important role in the formation of extracellular enzymes, DsbA is important in lipase stability and excretion | Pseudomonas aeruginosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 22000, DsbA, SDS-PAGE, 2 * 25000, DsbC, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
DsbA | - |
Pseudomonas aeruginosa |
DsbC | - |
Pseudomonas aeruginosa |