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Literature summary for 5.3.4.1 extracted from

  • Gilbert, H.F.
    Protein disulfide isomerase (1998), Methods Enzymol., 290, 26-50.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
insulin-(SS)
-
Rattus norvegicus
17
-
GSH
-
Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55114
-
x * 55114, calculation from nucleotide sequence Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bos taurus
-
?
-
?
additional information Mammalia protein disulfide isomerase serves as a subunit of at least two enzymes, the beta-subunit of the enzyme prolyl hydroxylase and an ER triglyceride transferase ?
-
?
Proteins Rattus norvegicus
-
?
-
?
Proteins Bos taurus
-
?
-
?
Proteins Mammalia when present in large stoichiometric excess relative to an unfolded protein substrate, the enzyme can exhibit chaperone activity, inhibiting aggregation and increasing the recovery of native protein ?
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Mammalia
-
-
-
Rattus norvegicus
-
-
-
Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Saccharomyces cerevisiae
glycoprotein
-
Bos taurus
no glycoprotein
-
Rattus norvegicus
no glycoprotein
-
Bos taurus

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Insulin-(SS) + GSH
-
Rattus norvegicus Insulin-(SH)2 + GSSG
-
?
Insulin-(SS) + GSH
-
Bos taurus Insulin-(SH)2 + GSSG
-
?
additional information
-
Bos taurus ?
-
?
additional information protein disulfide isomerase serves as a subunit of at least two enzymes, the beta-subunit of the enzyme prolyl hydroxylase and an ER triglyceride transferase Mammalia ?
-
?
Proteins native, reduced or with wrong disulfide bonds Mammalia Proteins with correct disulfide bonds ?
Proteins native, reduced or with wrong disulfide bonds Rattus norvegicus Proteins with correct disulfide bonds ?
Proteins native, reduced or with wrong disulfide bonds Saccharomyces cerevisiae Proteins with correct disulfide bonds ?
Proteins native, reduced or with wrong disulfide bonds Bos taurus Proteins with correct disulfide bonds ?
Proteins incorrectly disulfide-linked RNAse Rattus norvegicus Proteins with correct disulfide bonds ?
Proteins
-
Rattus norvegicus ?
-
?
Proteins
-
Bos taurus ?
-
?
Proteins when present in large stoichiometric excess relative to an unfolded protein substrate, the enzyme can exhibit chaperone activity, inhibiting aggregation and increasing the recovery of native protein Mammalia ?
-
?

Subunits

Subunits Comment Organism
?
-
Bos taurus
? x * 55114, calculation from nucleotide sequence Rattus norvegicus