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Literature summary for 5.3.3.8 extracted from

  • Yu, W.; Chu, X.; Deng, G.; Liu, X.; Chen, G.; Li, D.
    Mutation of Lys242 allows DELTA3-DELTA2-enoyl-CoA isomerase to acquire enoyl-CoA hydratase activity (2006), Biochim. Biophys. Acta, 1760, 1874-1883.
    View publication on PubMed
Search for more literature for 5.3.3.8

Cloned(Commentary)

Cloned (Comment) Organism
-
 Rattus norvegicus
expression in Escherichia coli Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
D149E decrease in catalytic activity Rattus norvegicus
D149E turnover number for trans-3-hexenoyl-CoA is 2.3fold lower than wild-type value, Km-value for trans-3-hexenoyl-CoA is fold higher than wild-type enzyme Rattus norvegicus
K242C decrease in catalytic activity, mutants shows additional hydratase activity on 2-hexenoyl-CoA with Vmax of 1.9 micromol per min and mg Rattus norvegicus
K242C turnover number for trans-3-hexenoyl-CoA is 14.5fold lower than wild-type value, Km-value for trans-3-hexenoyl-CoA is fold higher than wild-type enzyme Rattus norvegicus
K242C turnover number for trans-3-hexenoyl-CoA is 160 fold lower than wild-type value, Km-value for trans-3-hexenoyl-CoA is fold higher than wild-type enzyme. Mutation allows DELTA3-DELTA2-enoyl-CoA isomerase to acquire enoyl-CoA hydratase activity Rattus norvegicus
K242D decrease in catalytic activity, mutants shows slight additional hydratase activity on 2-hexenoyl-CoA with Vmax of 0.026 micromol per min and mg Rattus norvegicus
K242D turnover number for trans-3-hexenoyl-CoA is 12308fold lower than wild-type value, Km-value for trans-3-hexenoyl-CoA is fold higher than wild-type enzyme Rattus norvegicus
K242D turnover number for trans-3-hexenoyl-CoA is 2667fold lower than wild-type value, Km-value for trans-3-hexenoyl-CoA is fold higher than wild-type enzyme Rattus norvegicus
K242F decrease in catalytic activity Rattus norvegicus
K242F turnover number for trans-3-hexenoyl-CoA is 1143fold lower than wild-type value, Km-value for trans-3-hexenoyl-CoA is fold higher than wild-type enzyme Rattus norvegicus
K242R decrease in catalytic activity Rattus norvegicus
K242R turnover number for trans-3-hexenoyl-CoA is 3.2fold lower than wild-type value, Km-value for trans-3-hexenoyl-CoA is fold higher than wild-type enzyme Rattus norvegicus
K242T decrease in catalytic activity, mutants shows additional hydratase activity on 2-hexenoyl-CoA with Vmax of 0.041 micromol per min and mg Rattus norvegicus
K242T turnover number for trans-3-hexenoyl-CoA is 7619fold lower than wild-type value, Km-value for trans-3-hexenoyl-CoA is fold higher than wild-type enzyme Rattus norvegicus
K242T turnover number for trans-3-hexenoyl-CoA is 842fold lower than wild-type value, Km-value for trans-3-hexenoyl-CoA is fold higher than wild-type enzyme Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00062
-
 trans-2-hexenoyl-CoA pH 8, hydratase activity, mutant enzyme K242T Rattus norvegicus
0.0026
-
 trans-2-hexenoyl-CoA pH 8, hydratase activity, mutant enzyme K242D Rattus norvegicus
0.03
-
 trans-3-hexenoyl-CoA 25°C, pH 7.5, mutant enzyme K242D Rattus norvegicus
0.03
-
 3-trans-hexenoyl-CoA mutant K242D, pH 8.0 Rattus norvegicus
0.048
-
 trans-3-hexenoyl-CoA 25°C, pH 7.5, wild-type enzyme Rattus norvegicus
0.048
-
 3-trans-hexenoyl-CoA wild-type, pH 8.0 Rattus norvegicus
0.076
-
 trans-3-hexenoyl-CoA 25°C, pH 7.5, mutant enzyme K242T Rattus norvegicus
0.076
-
 3-trans-hexenoyl-CoA mutant K242T, pH 8.0 Rattus norvegicus
0.08
-
 trans-3-hexenoyl-CoA 25°C, pH 7.5, mutant enzyme D149E Rattus norvegicus
0.08
-
 3-trans-hexenoyl-CoA mutant D149E, pH 8.0 Rattus norvegicus
0.089
-
 trans-3-hexenoyl-CoA 25°C, pH 7.5, mutant enzyme K242R Rattus norvegicus
0.089
-
 3-trans-hexenoyl-CoA mutant K242R, pH 8.0 Rattus norvegicus
0.1
-
 trans-3-hexenoyl-CoA 25°C, pH 7.5, mutant enzyme K242F Rattus norvegicus
0.1
-
 3-trans-hexenoyl-CoA mutant K242F, pH 8.0 Rattus norvegicus
0.12
-
 trans-3-hexenoyl-CoA 25°C, pH 7.5, mutant enzyme K242C Rattus norvegicus
0.12
-
 3-trans-hexenoyl-CoA mutant K242C, pH 8.0 Rattus norvegicus
0.31
-
 trans-2-hexenoyl-CoA pH 8, hydratase activity, mutant enzyme K242C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus one of the key enzymes involved in fatty acid oxidation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-
Rattus norvegicus P23965
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-trans-Hexenoyl-CoA
-
 Rattus norvegicus 2-trans-Hexenoyl-CoA
-
 ?
additional information one of the key enzymes involved in fatty acid oxidation Rattus norvegicus ?
-
 ?
trans-2-hexenoyl-CoA + H2O K242C mutation allows Delta3-Delta2-enoyl-CoA isomerase to acquire enoyl-CoA hydratase activity Rattus norvegicus 3-hydroxyhexanoyl-CoA
-
 ?
trans-3-hexenoyl-CoA
-
 Rattus norvegicus trans-2-hexenoyl-CoA
-
 ?

Synonyms

Synonyms Comment Organism
Delta3-Delta2-enoyl-CoA isomerase
-
 Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.013
-
 trans-2-hexenoyl-CoA pH 8, hydratase activity, mutant enzyme K242D Rattus norvegicus
0.021
-
 trans-2-hexenoyl-CoA pH 8, hydratase activity, mutant enzyme K242T Rattus norvegicus
0.06
-
 trans-3-hexenoyl-CoA 25°C, pH 7.5, mutant enzyme K242D Rattus norvegicus
0.06
-
 3-trans-hexenoyl-CoA mutant K242D, pH 8.0 Rattus norvegicus
0.14
-
 trans-3-hexenoyl-CoA 25°C, pH 7.5, mutant enzyme K242F Rattus norvegicus
0.14
-
 3-trans-hexenoyl-CoA mutant K242F, pH 8.0 Rattus norvegicus
0.19
-
 trans-3-hexenoyl-CoA 25°C, pH 7.5, mutant enzyme K242T Rattus norvegicus
0.19
-
 3-trans-hexenoyl-CoA mutant K242T, pH 8.0 Rattus norvegicus
1
-
 trans-2-hexenoyl-CoA pH 8, hydratase activity, mutant enzyme K242C Rattus norvegicus
11
-
 trans-3-hexenoyl-CoA 25°C, pH 7.5, mutant enzyme K242C Rattus norvegicus
11
-
 3-trans-hexenoyl-CoA mutant K242C, pH 8.0 Rattus norvegicus
50
-
 trans-3-hexenoyl-CoA 25°C, pH 7.5, mutant enzyme K242R Rattus norvegicus
50
-
 3-trans-hexenoyl-CoA mutant K242R, pH 8.0 Rattus norvegicus
70
-
 trans-3-hexenoyl-CoA 25°C, pH 7.5, mutant enzyme D149E Rattus norvegicus
70
-
 3-trans-hexenoyl-CoA mutant D149E, pH 8.0 Rattus norvegicus
160
-
 3-trans-hexenoyl-CoA wild-type, pH 8.0 Rattus norvegicus
160
-
 trans-3-hexenoyl-CoA 25°C, pH 7.5, wild-type enzyme Rattus norvegicus