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Literature summary for 5.3.3.8 extracted from

  • Muller-Newen, G.; Stoffel, W.
    Site-directed mutagenesis of putative active-site amino acid residues of 3,2-trans-enoyl-CoA isomerase, conserved within the low-homology isomerase/hydratase enzyme family (1993), Biochemistry, 32, 11405-11412.
    View publication on PubMed
Search for more literature for 5.3.3.8

Cloned(Commentary)

Cloned (Comment) Organism
wild type and mutant enzymes expressed in Escherichia coli Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
E151X site-directe mutagenesis of putative active-site amino acid residues. Exchange of Arg151 and Asp211 leads to a reduced expression of the recombinant enzyme accompanied by a reduced activity. The replacement of Glu165 by Gln leads to a strongly reduced enzymatic activity. Tyr150 is not involved in isomerase catalysis Rattus norvegicus
E165Q site-directe mutagenesis of putative active-site amino acid residues. Exchange of Arg151 and Asp211 leads to a reduced expression of the recombinant enzyme accompanied by a reduced activity. The replacement of Glu165 by Gln leads to a strongly reduced enzymatic activity. Tyr150 is not involved in isomerase catalysis Rattus norvegicus
N211X site-directe mutagenesis of putative active-site amino acid residues. Exchange of Arg151 and Asp211 leads to a reduced expression of the recombinant enzyme accompanied by a reduced activity. The replacement of Glu165 by Gln leads to a strongly reduced enzymatic activity. Tyr150 is not involved in isomerase catalysis Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion enzyme is synthesized with a N-terminal mitochondrial targeting sequence which is cleaved during mitochondrial import Rattus norvegicus 5739
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus key enzyme in the degradation of unsaturated fatty acids ?
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 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information key enzyme in the degradation of unsaturated fatty acids Rattus norvegicus ?
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 ?