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Literature summary for 5.3.3.8 extracted from

  • Miscowicz, F.M.; Bloch, K.
    Purification of hog liver isomerase. Mechanism of isomerization of 3-alkenyl and 3-alkynyl thioesters (1979), J. Biol. Chem., 254, 5868-5877.
    View publication on PubMed
Search for more literature for 5.3.3.8

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Sus scrofa
0.04
-
 3-hexenoyl-CoA pI 7.01 enzyme Sus scrofa
0.09
-
 3-trans-hexenoyl-CoA pI 7.01 enzyme Sus scrofa
0.11
-
 3-trans-hexenoyl-CoA pI 6.83 enzyme Sus scrofa
0.14
-
 3-cis-hexenoyl-CoA pI 7.01 enzyme Sus scrofa
0.15
-
 3-hexenoyl-CoA pI 6.83 enzyme Sus scrofa
0.24
-
 3-decynoyl-N-acetylcysteamine pI 7.01 enzyme Sus scrofa
0.27
-
 3-cis-hexenoyl-CoA pI 6.83 enzyme Sus scrofa
0.28
-
 3-dodecynoyl-N-acetylcysteamine pI 7.01 enzyme Sus scrofa
0.5
-
 3-dodecynoyl-N-acetylcysteamine pI 6.57 enzyme Sus scrofa
0.52
-
 3-decynoyl-N-acetylcysteamine pI 6.83 enzyme Sus scrofa
0.68
-
 3-octynoyl-N-acetylcysteamine pI 7.01 enzyme Sus scrofa
0.81
-
 3-decynoyl-N-acetylcysteamine pI 6.57 enzyme Sus scrofa
0.97
-
 3-dodecynoyl-N-acetylcysteamine pI 6.83 enzyme Sus scrofa
1
-
 3-octynoyl-N-acetylcysteamine pI 6.57 enzyme Sus scrofa
1.6
-
 3-octynoyl-N-acetylcysteamine pI 6.83 enzyme Sus scrofa
2.1
-
 3-hexynoyl-N-acetylcysteamine pI 7.01 enzyme Sus scrofa
4
-
 3-hexynoyl-N-acetylcysteamine pI 6.57 enzyme Sus scrofa
7.2
-
 3-hexynoyl-N-acetylcysteamine pI 6.83 enzyme Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 2 * 47000, SDS-PAGE Sus scrofa
60000
-
 gel filtration Sus scrofa
91000
-
 equilibrium ultracentrifugation Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
 hog
-

Purification (Commentary)

Purification (Comment) Organism
4 enzyme forms: pI 7.27 enzyme, pI 7.01 enzyme, pI 6.83 enzyme, pI 6.57 enzyme Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-cis-Hexenoyl-CoA
-
 Sus scrofa ?
-
 ?
3-Decenoyl-pantetheine
-
 Sus scrofa ?
-
 ?
3-Decynoyl-CoA
-
 Sus scrofa ?
-
 ?
3-decynoyl-N-acetylcysteamine
-
 Sus scrofa 2,3-decadienoyl-N-acetylcysteamine
-
 ?
3-dodecynoyl-N-acetylcysteamine r Sus scrofa ?
-
 ?
3-Hexynoyl-CoA
-
 Sus scrofa ?
-
 ?
3-hexynoyl-N-acetylcysteamine
-
 Sus scrofa ?
-
 ?
3-octynoyl-N-acetylcysteamine
-
 Sus scrofa ?
-
 ?
3-trans-Hexenoyl-CoA
-
 Sus scrofa 2-trans-Hexenoyl-CoA
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 47000, SDS-PAGE Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Sus scrofa
3
-
 3-trans-hexenoyl-CoA pI 6.83 enzyme Sus scrofa
4
-
 3-cis-hexenoyl-CoA pI 6.83 enzyme Sus scrofa
5
-
 3-trans-hexenoyl-CoA pI 7.01 enzyme Sus scrofa
9.4
-
 3-cis-hexenoyl-CoA pI 7.01 enzyme Sus scrofa
12.1
-
 3-hexynoyl-CoA pI 7.01 enzyme Sus scrofa
25
-
 3-hexynoyl-CoA pI 6.83 enzyme Sus scrofa
33
-
 3-hexynoyl-N-acetylcysteamine pI 6.57 enzyme Sus scrofa
50
-
 3-decynoyl-N-acetylcysteamine pI 6.57 enzyme Sus scrofa
59
-
 3-dodecynoyl-N-acetylcysteamine pI 6.83 enzyme Sus scrofa
61
-
 3-hexynoyl-N-acetylcysteamine pI 6.83 enzyme Sus scrofa
68
-
 3-decynoyl-N-acetylcysteamine pI 6.57 enzyme Sus scrofa
105
-
 3-octynoyl-N-acetylcysteamine pI 7.01 enzyme Sus scrofa
128
-
 3-decynoyl-N-acetylcysteamine pI 7.01 enzyme Sus scrofa
130
-
 3-hexynoyl-N-acetylcysteamine pI 6.83 enzyme Sus scrofa
160
-
 3-dodecynoyl-N-acetylcysteamine pI 7.01 enzyme Sus scrofa
190
-
 3-decynoyl-N-acetylcysteamine pI 6.83 enzyme Sus scrofa
284
-
 3-decynoyl-N-acetylcysteamine pI 6.83 enzyme Sus scrofa
500
-
 3-dodecynoyl-N-acetylcysteamine pI 6.83 enzyme Sus scrofa