Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 177% activity at 1 mM | Pseudomonas sp. WU-0701 | |
dithiothreitol | 166% activity at 1 mM | Pseudomonas sp. WU-0701 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 9% residual activity at 1 mM | Pseudomonas sp. WU-0701 | |
EDTA | 84% residual activity at 1 mM | Pseudomonas sp. WU-0701 | |
glycerol | 78% residual activity at 20% (v/v) | Pseudomonas sp. WU-0701 | |
Hg2+ | 5% residual activity at 1 mM | Pseudomonas sp. WU-0701 | |
Mo4+ | 58% residual activity at 1 mM | Pseudomonas sp. WU-0701 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.9 | - |
cis-aconitate | at pH 6.0 and 37°C | Pseudomonas sp. WU-0701 | |
8 | - |
trans-aconitate | at pH 6.0 and 37°C | Pseudomonas sp. WU-0701 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 169% activity at 1 mM | Pseudomonas sp. WU-0701 | |
Cu2+ | 147% activity at 1 mM | Pseudomonas sp. WU-0701 | |
Fe2+ | 177% activity at 1 mM | Pseudomonas sp. WU-0701 | |
Mg2+ | 183% activity at 1 mM | Pseudomonas sp. WU-0701 | |
Mn2+ | 160% activity at 1 mM | Pseudomonas sp. WU-0701 | |
Ni2+ | 157% activity at 1 mM | Pseudomonas sp. WU-0701 | |
Zn2+ | 128% activity at 1 mM | Pseudomonas sp. WU-0701 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25300 | - |
gel filtration | Pseudomonas sp. WU-0701 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-aconitate | Pseudomonas sp. WU-0701 | - |
trans-aconitate | - |
r | |
trans-Aconitate | Pseudomonas sp. WU-0701 | - |
cis-Aconitate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. WU-0701 | A0A0A1H8I4 | - |
- |
Purification (Comment) | Organism |
---|---|
CM-Sepharose column chromatography, Bio-Scale CHT5-I column chromatography, and Superdex 200 gel filtration | Pseudomonas sp. WU-0701 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.16 | - |
cell-free extract, at pH 7.0 and 37°C | Pseudomonas sp. WU-0701 |
1267 | - |
after 305fold purification, at pH 7.0 and 37°C | Pseudomonas sp. WU-0701 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-aconitate | - |
Pseudomonas sp. WU-0701 | trans-aconitate | - |
r | |
additional information | no activity with citric acid, succinic acid, fumaric acid, and 2-oxoglutaric acid, and other dicarboxylic acids, such as itaconic acid, formic acid, citraconic acid, and maleic acid | Pseudomonas sp. WU-0701 | ? | - |
? | |
trans-Aconitate | - |
Pseudomonas sp. WU-0701 | cis-Aconitate | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 25000, SDS-PAGE | Pseudomonas sp. WU-0701 |
Synonyms | Comment | Organism |
---|---|---|
Aconitate isomerase | - |
Pseudomonas sp. WU-0701 |
ais | - |
Pseudomonas sp. WU-0701 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Pseudomonas sp. WU-0701 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | about 50% activity at 20°C, about 90-100% activity at 30-37°C, about 70% activity at 40°C, about 35% activity at 50°C, about 20% activity at 60 and 70°C | Pseudomonas sp. WU-0701 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4500 | - |
cis-aconitate | at pH 6.0 and 37°C | Pseudomonas sp. WU-0701 | |
15000 | - |
trans-aconitate | at pH 6.0 and 37°C | Pseudomonas sp. WU-0701 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Pseudomonas sp. WU-0701 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | - |
more than 50% activity between pH 4.0 and 6.0 | Pseudomonas sp. WU-0701 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not require any cofactors such as NADH and NADPH | Pseudomonas sp. WU-0701 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
190 | - |
trans-aconitate | at pH 6.0 and 37°C | Pseudomonas sp. WU-0701 | |
760 | - |
cis-aconitate | at pH 6.0 and 37°C | Pseudomonas sp. WU-0701 |