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Literature summary for 5.3.3.5 extracted from

  • Rahier, A.; Pierre, S.; Riveill, G.; Karst, F.
    Identification of essential amino acid residues in a sterol 8,7-isomerase from Zea mays reveals functional homology and diversity with the isomerases of animal and fungal origin (2008), Biochem. J., 414, 247-259.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in the Saccharomyces cerevisiae mutant erg2 Zea mays

Protein Variants

Protein Variants Comment Organism
D107V inactive Zea mays
E116V mutant exhibits 72% of wild type activity Saccharomyces cerevisiae
E121V inactive Zea mays
E73V mutant exhibits 100% of wild type activity Saccharomyces cerevisiae
E78V inactive Zea mays
H69L mutant exhibits 70% of wild type activity Saccharomyces cerevisiae
H74L inactive Zea mays
T119I inactive Saccharomyces cerevisiae
T124I mutant exhibits 10% of wild type activity Zea mays
W193L inactive Zea mays
W66L inactive Zea mays

Inhibitors

Inhibitors Comment Organism Structure
AY9944
-
Zea mays
fenpropimorph
-
Saccharomyces cerevisiae
fenpropimorph
-
Zea mays
additional information not inhibited by N-benzamido-decalin, tamoxifen, N-benzyl-8-azadecalin, and AY9944 Saccharomyces cerevisiae
additional information not inhibited by N-benzamido-decalin Zea mays
SR31747
-
Saccharomyces cerevisiae
SR31747
-
Zea mays
tamoxifen
-
Zea mays
tridemorph
-
Saccharomyces cerevisiae
tridemorph
-
Zea mays
Trifluoperazine
-
Saccharomyces cerevisiae
Trifluoperazine
-
Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.116
-
stigmasta-8-en-3beta-ol
-
Zea mays
0.512
-
cholesta-8,24-dien-3beta-ol
-
Zea mays
0.522
-
4alpha-methyl-ergosta-8,24-dien-3beta-ol
-
Zea mays

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Zea mays
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4alpha-methyl-ergosta-8,24-dien-3beta-ol
-
Zea mays ?
-
?
4alpha-methyl-fecosterol
-
Zea mays 4alpha-methyl-ergosta-7,24-dien-3beta-ol
-
?
cholesta-8,24-dien-3beta-ol
-
Zea mays ?
-
?
DELTA8-sitostenol
-
Zea mays stigmast-7-en-3beta-ol
-
?
additional information 4,4-dimethyl-cholesta-8,24-dien-3beta-ol, 4,4-dimethyl-ergosta-8,24-dien-3beta-ol, 4alpha,14aloha-dimethyl-ergosta-8,24-dien-3beta-ol, and cholest-8(14)-en-3beta-ol are no substrates Zea mays ?
-
?
stigmasta-8-en-3beta-ol
-
Zea mays ?
-
?
zymosterol
-
Saccharomyces cerevisiae cholesta-7,24-dienol
-
?
zymosterol
-
Zea mays cholesta-7,24-dienol
-
?

Synonyms

Synonyms Comment Organism
8,7SI
-
Saccharomyces cerevisiae
8,7SI
-
Zea mays
EBP
-
Zea mays
ERG2p
-
Saccharomyces cerevisiae
sterol 8,7-isomerase
-
Saccharomyces cerevisiae
sterol 8,7-isomerase
-
Zea mays

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00003
-
-
Saccharomyces cerevisiae fenpropimorph
0.0001
-
-
Zea mays AY9944
0.0001
-
-
Zea mays SR31747
0.00021
-
-
Zea mays fenpropimorph
0.0006
-
-
Saccharomyces cerevisiae SR31747
0.00085
-
-
Zea mays tridemorph
0.001
-
-
Zea mays Trifluoperazine
0.001
-
-
Saccharomyces cerevisiae tridemorph
0.0085
-
-
Zea mays tamoxifen
0.5
-
IC50 above 0.5 mM Saccharomyces cerevisiae Trifluoperazine