Literature summary for 5.3.3.21 extracted from

Filppula, S.A.; Yagi, A.I.; Kilpelaeinen, S.H.; Novikov, D.; FitzPatrick, D.R.; Vihinen, M.; Valle, D.; Hiltunen, J.K.
DELTA3,5-DELTA2,4-dienoyl-CoA isomerase from rat liver. Molecular characterization (1998), J. Biol. Chem., 273, 349-355 .

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Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Rattus norvegicus	5739	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
170000	-	gel filtration	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
a (3E,5Z)-alka-3,5-dienoyl-CoA	Rattus norvegicus	-	a (2E,4E)-alka-2,4-dienoyl-CoA	-	?

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	P14604	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE-Sephacel column chromatography, Resource S column chromatography, and Superdex 200 gel filtration	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.06	-	with 3,5,8,11,14-eicosapentaenoyl-CoA as substrate, pH and temperature not specified in the publication	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
a (3E,5Z)-alka-3,5-dienoyl-CoA	-	Rattus norvegicus	a (2E,4E)-alka-2,4-dienoyl-CoA	-	?
additional information	the enzyme also has a low 2-enoyl-CoA hydratase 1 (crotonase) activity, generating L-3-hydroxydecanoyl-CoA from trans-2-decenoyl-CoA. The enzyme has no activity with trans-3-decenoyl-CoA	Rattus norvegicus	?	-	?

Subunits

Subunits	Comment	Organism
homohexamer	6 * 32000, mature mitochondrial enzyme form, SDS-PAGE	Rattus norvegicus
homohexamer	6 * 36000, peroxisomal enzyme form, SDS-PAGE	Rattus norvegicus

Synonyms

Synonyms	Comment	Organism
DELTA3,5-DELTA2,4-dienoyl-CoA isomerase	-	Rattus norvegicus
ECH1p	-	Rattus norvegicus
enoyl-CoA hydratase	-	Rattus norvegicus

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Release 2023.1 (January 2023)