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Literature summary for 5.3.3.21 extracted from
- DELTA3,5,DELTA2,4-dienoyl-CoA isomerase from rat liver mitochondria purification and characterization of a new enzyme involved in the beta-oxidation of unsaturated fatty acids (1994), J. Biol. Chem., 269, 2384-2388 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0075 | - |
3,5-cis-octadienoyl-CoA | at pH 8.0 and 25°C | Rattus norvegicus |  |
| 0.0118 | - |
3,5-trans-octadienoyl-CoA | at pH 8.0 and 25°C | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 126000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| a (3E,5Z)-alka-3,5-dienoyl-CoA | Rattus norvegicus | - |
a (2E,4E)-alka-2,4-dienoyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| PEG precipitation, Q Sepharo column chromatography, Sepharose CL-6B column chromatography, and hydroxylapatite column chromatography | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,5-cis-octadienoyl-CoA | best substrate | Rattus norvegicus | 2,4-octadienoyl-CoA | - |
? | |
| 3,5-trans-octadienoyl-CoA | - |
Rattus norvegicus | 2,4-octadienoyl-CoA | - |
? | |
| a (3E,5Z)-alka-3,5-dienoyl-CoA | - |
Rattus norvegicus | a (2E,4E)-alka-2,4-dienoyl-CoA | - |
? | |
| additional information | no activity with 2-trans-5-trans-octadienoyl-CoA | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 32000, SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DELTA3,5,DELTA2,4-dienoyl-CoA isomerase | - |
Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | half-maximal activities at pH 5.0 and 10.0 | Rattus norvegicus |
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