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Literature summary for 5.3.3.2 extracted from

  • Chen, H.; Li, M.; Liu, C.; Zhang, H.; Xian, M.; Liu, H.
    Enhancement of the catalytic activity of isopentenyl diphosphate isomerase (IDI) from Saccharomyces cerevisiae through random and site-directed mutagenesis (2018), Microb. Cell Fact., 17, 65 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
L141H the mutation enhances the enzyme activity 1.1fold Saccharomyces cerevisiae
L141H/W256C the mutations enhance the enzyme activity 1.65fold Saccharomyces cerevisiae
L141H/Y195F the mutations enhance the enzyme activity 2.03fold Saccharomyces cerevisiae
L141H/Y195F/W256C the mutation enhances the enzyme activity 3.13fold Saccharomyces cerevisiae
W256C the mutation enhances the enzyme activity 0.47fold Saccharomyces cerevisiae
Y195F the mutation enhances the enzyme activity 0.71fold Saccharomyces cerevisiae
Y195F/W256C the mutations enhance the enzyme activity 1.14fold Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0376
-
 isopentenyl diphosphate mutant enzyme L141H/Y195F/W256C, at pH 7.5 and 30°C Saccharomyces cerevisiae
0.0415
-
 isopentenyl diphosphate wild type enzyme, at pH 7.5 and 30°C Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isopentenyl diphosphate Saccharomyces cerevisiae
-
 dimethylallyl diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P15496
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isopentenyl diphosphate
-
 Saccharomyces cerevisiae dimethylallyl diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
IDI
-
 Saccharomyces cerevisiae
isopentenyl diphosphate isomerase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.27
-
 isopentenyl diphosphate wild type enzyme, at pH 7.5 and 30°C Saccharomyces cerevisiae
10.57
-
 isopentenyl diphosphate mutant enzyme L141H/Y195F/W256C, at pH 7.5 and 30°C Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
200
-
 isopentenyl diphosphate wild type enzyme, at pH 7.5 and 30°C Saccharomyces cerevisiae
280
-
 isopentenyl diphosphate mutant enzyme L141H/Y195F/W256C, at pH 7.5 and 30°C Saccharomyces cerevisiae