Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15pREP4 cells | Streptococcus pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
N37A | the mutant is a monomeric but active enzyme with by 20°C lowered melting temperature and reduced binding affinities of FMN (40fold) and a minimal effect on the kcat value compared to the wild type enzyme | Streptococcus pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Streptococcus pneumoniae | |
isopentenyl diphosphate | - |
Streptococcus pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00595 | - |
isopentenyl diphosphate | wild type enzyme, at anaerobic conditions, at pH 7.0 and 37°C | Streptococcus pneumoniae | |
0.0408 | - |
isopentenyl diphosphate | wild type enzyme, at aerobic conditions, at pH 7.0 and 37°C | Streptococcus pneumoniae | |
0.572 | - |
isopentenyl diphosphate | mutant enzyme N37A, at aerobic conditions, at pH 7.0 and 37°C | Streptococcus pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isopentenyl diphosphate | Streptococcus pneumoniae | - |
dimethylallyl diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose resin column chromatography | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isopentenyl diphosphate | - |
Streptococcus pneumoniae | dimethylallyl diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Streptococcus pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
Idi-2 | - |
Streptococcus pneumoniae |
type 2 isopentenyl diphosphate:dimethylallyl diphosphate isomerase | - |
Streptococcus pneumoniae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.875 | - |
isopentenyl diphosphate | wild type enzyme, at anaerobic conditions, at pH 7.0 and 37°C | Streptococcus pneumoniae | |
0.983 | - |
isopentenyl diphosphate | wild type enzyme, at aerobic conditions, at pH 7.0 and 37°C | Streptococcus pneumoniae | |
1.31 | - |
isopentenyl diphosphate | mutant enzyme N37A, at aerobic conditions, at pH 7.0 and 37°C | Streptococcus pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Streptococcus pneumoniae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0064 | - |
FMN | wild type enzyme, at anaerobic conditions, at pH 7.0 and 37°C | Streptococcus pneumoniae | |
0.292 | - |
isopentenyl diphosphate | wild type enzyme, at aerobic conditions, at pH 7.0 and 37°C | Streptococcus pneumoniae |