Literature summary for 5.3.3.2 extracted from

Hoshino, T.; Nango, E.; Baba, S.; Eguchi, T.; Kumasaka, T.
Crystallization and preliminary X-ray analysis of isopentenyl diphosphate isomerase from Methanocaldococcus jannaschii (2011), Acta Crystallogr. Sect. F, 67, 101-103.

Search for more literature for 5.3.3.2

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Methanocaldococcus jannaschii

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 0.6 M calcium acetate and 50 mM HEPES pH 7.5	Methanocaldococcus jannaschii

Inhibitors

Inhibitors	Comment	Organism	Structure
3,4-epoxy-3-methylbutyl diphosphate	irreversible inhibition	Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	IDI-2 requires a divalent cation such as Mg2+ for activity	Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	x * 40000, SDS-PAGE	Methanocaldococcus jannaschii

Organism

Organism	UniProt	Comment	Textmining
Methanocaldococcus jannaschii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE Sepharose column chromatography and Resource Phe column chromatograpyh	Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
isopentenyl diphosphate	-	Methanocaldococcus jannaschii	dimethylallyl diphosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 40000, SDS-PAGE	Methanocaldococcus jannaschii

Synonyms

Synonyms	Comment	Organism
Idi-2	-	Methanocaldococcus jannaschii
type 2 isopentenyl diphosphate isomerase	-	Methanocaldococcus jannaschii

Cofactor

Cofactor	Comment	Organism	Structure
FMN	required, IDI-2 reconstituted with cofactor analogues such as 5-deaza-FMN show no activity	Methanocaldococcus jannaschii

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Release 2023.1 (January 2023)