Literature summary for 5.3.3.2 extracted from

Lee, S.; Poulter, C.D.
Escherichia coli type I isopentenyl diphosphate isomerase: structural and catalytic roles for divalent metals (2006), J. Am. Chem. Soc., 128, 11545-11550.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals soaked with transition state analogue (N,N-dimethylamino)-1-ethyl diphosphate	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
(N,N-dimethylamino)-1-ethyl diphosphate	transition state analogue	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cd2+	reconstitution of metal-free enzyme with Mg2+, Mn2+, Zn2+, Co2+, Ni2+ or Cd2+ generates active enzyme	Escherichia coli
Co2+	reconstitution of metal-free enzyme with Mg2+, Mn2+, Zn2+, Co2+, Ni2+ or Cd2+ generates active enzyme	Escherichia coli
Mg2+	0.72 mol per mol of enzyme. Reconstitution of metal-free enzyme with Mg2+, Mn2+, Zn2+, Co2+, Ni2+ or Cd2+ generates active enzyme	Escherichia coli
Mn2+	0.10 mol per mol of enzyme. Reconstitution of metal-free enzyme with Mg2+, Mn2+, Zn2+, Co2+, Ni2+ or Cd2+ generates active enzyme	Escherichia coli
Ni2+	reconstitution of metal-free enzyme with Mg2+, Mn2+, Zn2+, Co2+, Ni2+ or Cd2+ generates active enzyme	Escherichia coli
Zn2+	0.92 mol per mol of enzyme. Reconstitution of metal-free enzyme with Mg2+, Mn2+, Zn2+, Co2+, Ni2+ or Cd2+ generates active enzyme	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	type I enzyme	-

Renatured (Commentary)

Renatured (Comment)	Organism
reconstitution of metal-free enzyme with Mg2+, Mn2+, Zn2+, Co2+, Ni2+ or Cd2+ generates active enzyme	Escherichia coli

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Release 2023.1 (January 2023)