BRENDA - Enzyme Database
show all sequences of 5.3.3.14

Mechanism of action of beta-hydroxydecanoyl thioester dehydrase

Rando, R.R.; Bloch, K.; J. Biol. Chem. 243, 5627-5634 (1968)

Data extracted from this reference:

Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
multifunctional enzyme, catalyzing in addition reaction of EC 4.2.1.60
-
Reaction
Reaction
Commentary
Organism
Reaction ID
a trans-dec-2-enoyl-[acyl-carrier protein] = a cis-dec-3-enoyl-[acyl-carrier protein]
reaction of EC4.2.1.60 and isomerization reaction, both catalyzed by the enzyme, share the common intermediate, enzyme-bound alpha,beta-decenoate
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
trans-2-decenoyl-N-acetylcysteamine
-
665451
Escherichia coli
cis-3-decenoyl-N-acetylcysteamine
-
-
-
r
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.95
-
assay at
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
trans-2-decenoyl-N-acetylcysteamine
-
665451
Escherichia coli
cis-3-decenoyl-N-acetylcysteamine
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-
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r
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.95
-
assay at
Escherichia coli
Other publictions for EC 5.3.3.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
665373
Fozo
The fabM gene product of Strep ...
Streptococcus mutans
J. Bacteriol.
186
4152-4158
2004
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1
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665480
Marrakchi
A new mechanism for anaerobic ...
Streptococcus pneumoniae
J. Biol. Chem.
277
44809-44816
2002
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2
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666947
Leesong
Structure of a dehydratase-iso ...
Escherichia coli
Structure
4
253-264
1996
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5819
Guerra
Escherichia coli beta-hydroxyd ...
Escherichia coli
Arch. Biochem. Biophys.
280
336-345
1990
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5814
Cronan
Derived amino acid sequence an ...
Escherichia coli
J. Biol. Chem.
263
4641-4646
1988
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5808
Endo
Mode of inhibition of beta-hyd ...
Escherichia coli
J. Biol. Chem.
245
4293-4296
1970
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1
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5803
Helmkamp
beta-Hydroxydecanoyl thioester ...
Escherichia coli
J. Biol. Chem.
244
6014-6022
1969
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1
1
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1
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1
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5807
Helmkamp
beta-Hydroxydecanoyl thioester ...
Escherichia coli
J. Biol. Chem.
243
3229-3231
1968
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4
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665451
Rando
Mechanism of action of beta-hy ...
Escherichia coli
J. Biol. Chem.
243
5627-5634
1968
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5800
Kass
beta-Hydroxydecanoyl thioester ...
Escherichia coli
J. Biol. Chem.
242
4418-4431
1967
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