Literature summary for 5.3.3.1 extracted from

Wilde, T.C.; Blotny, G.; Pollack, R.M.
Experimental evidence for enzyme-enhanced coupled motion/quantum mechanical hydrogen tunneling by ketosteroid isomerase (2008), J. Am. Chem. Soc., 130, 6577-6585.

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Protein Variants

Protein Variants	Comment	Organism
D99A	secondary kinetic isotope effects similar to wild-type. Mutation does not significantly decrease the contribution of coupled motion/hydrogen tunneling to the enzymatic reaction	Comamonas testosteroni
Y14F	secondary kinetic isotope effects similar to wild-type. Mutation does not significantly decrease the contribution of coupled motion/hydrogen tunneling to the enzymatic reaction	Comamonas testosteroni

Organism

Organism	UniProt	Comment	Textmining
Comamonas testosteroni	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
a 3-oxo-DELTA5-steroid = a 3-oxo-DELTA4-steroid	enzyme enhances the coupled motion/hydrogen tunneling contribution to the rate acceleration over the solution reaction	Comamonas testosteroni	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
androst-5-ene-3,17-dione	alpha-secondary deuterium kinetic isotope effects at C6 of the steroid. Presence of coupled motion/hydrogen tunneling in the enzyme-catalyzed reaction	Comamonas testosteroni	androst-4-ene-3,17-dione	-	r

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Release 2023.1 (January 2023)