Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.3.1 extracted from

  • Jang do, S.; Cha, H.J.; Cha, S.S.; Hong, B.H.; Ha, N.C.; Lee, J.Y.; Oh, B.H.; Lee, H.S.; Choi, K.Y.
    Structural double-mutant cycle analysis of a hydrogen bond network in ketosteroid isomerase from Pseudomonas putida biotype B (2004), Biochem. J., 382, 967-973.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant Y14F/D99L, increase in hydrophobic interaction while disrupting the hydrogen bond network, mutants Y30F/D99L and Y55F/D99L, disruption of hydrogen bond network Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
Y14F/D99L partly additive effect of mutations for both, catalysis and stability, increase in hydrophobic interaction while disrupting the hydrogen bond network, crystallization data Pseudomonas putida
Y30F/D99L disruption of hydrogen bond network, crystallization data Pseudomonas putida
Y55F/D99L disruption of hydrogen bond network, crystallization data Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
biotype B
-