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Literature summary for 5.3.2.6 extracted from

  • Whitman, C.; Hajipour, G.; Watson, R.; Johnson Jr., W.; Bembenek, M.; Stolowich, N.
    Stereospecific ketonization of 2-hydroxymuconate by 4-oxalocrotonate tautomerase and 5-(carboxymethyl)-2-hydroxymuconate isomerase (1992), J. Am. Chem. Soc., 114, 10104-10110.
No PubMed abstract available
Search for more literature for 5.3.2.6

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
 x * 14000, 5-(carboxymethyl)-2-hydroxymuconate isomerase, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli C
-
-
-
Pseudomonas putida
-
-
-
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from Escherichia coli strain C by ammonium sulfate fractionation, gel filtration, anion exchange chromatography, ultrafiltration, ammoniums ulfate fractionation, and hydrophobic interaction chromatography, followed by gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxymuconate stereospecific ketonization Pseudomonas putida 2-oxo-3-(E)-hexenedioate
-
 ?
2-hydroxymuconate stereospecific ketonization, a reaction of EC 5.3.2.6, is also catalyzed by 5-(carboxymethyl)-2-hydroxymuconate isomerase, EC 5.3.3.10 Escherichia coli 2-oxo-3-(E)-hexenedioate
-
 ?
2-hydroxymuconate stereospecific ketonization Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 2-oxo-3-(E)-hexenedioate
-
 ?
2-hydroxymuconate stereospecific ketonization, a reaction of EC 5.3.2.6, is also catalyzed by 5-(carboxymethyl)-2-hydroxymuconate isomerase, EC 5.3.3.10 Escherichia coli C 2-oxo-3-(E)-hexenedioate
-
 ?

Subunits

Subunits Comment Organism
? x * 14000, 5-(carboxymethyl)-2-hydroxymuconate isomerase, SDS-PAGE Escherichia coli
? x * 3600, 4-oxalocrotonate tautomerase, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
4-OT
-
 Pseudomonas putida
4-oxalocrotonate tautomerase
-
 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Escherichia coli
30
-
 assay at Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
 assay at Escherichia coli
7.3
-
 assay at Pseudomonas putida

General Information

General Information Comment Organism
evolution mechanism and the evolution of 4-oxalocrotonate tautomerase, and 5-(carboxymethyl)-2-hydroxymuconate isomerase, EC 5.3.3.10, and their respective pathways, overview Pseudomonas putida
evolution mechanism and the evolution of 4-oxalocrotonate tautomerase, EC 5.3.2.6, and 5-(carboxymethyl)-2-hydroxymuconate isomerase and their respective pathways, overview Escherichia coli
metabolism in the catechol meta-fission pathway elaborated by Pseudomonas putida mt-2 ketonization of 2-hydroxymuconate by 4-oxalocrotonate tautomerase generates the alpha,beta-unsaturated ketone 2-oxo-3-(E)-hexenedioate, which undergoes decarboxylation and further processing to intermediates in the Krebs cycle Pseudomonas putida
metabolism in the homoprotocatechuate pathway elaborated by Escherichia coli C ketonization of 5-(carboxymethyl)-2-hydroxymuconate by 5-(carboxymethyl)-2-hydroxymuconate isomerase generates the alpha,beta-unsaturated ketone, which undergoes decarboxylation and further processing to intermediates in the Krebs cycle Escherichia coli