Literature summary for 5.3.2.6 extracted from

Subramanya, H.S.; Roper, D.I.; Dauter, Z.; Dodson, E.J.; Davies, G.J.; Wilson, K.S.; Wigley, D.B.
Enzymatic ketonization of 2-hydroxymuconate: specificity and mechanism investigated by the crystal structures of two isomerases (1996), Biochemistry, 35, 792-802.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
4-OT from 80% saturated ammonium sulfate solution containing 100 mM HEPES, pH 7.5, and 1% PEG 200, 20°C, X-ray diffraction structure determination and analysis at 1.9-2.8 A resolution, heavy etal derivatization by soaking the crystals for 4 h in crystallization solution containing 2 mM K2PtCl4	Pseudomonas sp.

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp.	P49172	-	-
Pseudomonas sp. CF 600	P49172	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate = (3E)-2-oxohex-3-enedioate	substrate specificity and one-base molecular mechanism, overview	Pseudomonas sp.	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	substrate specificity, overview	Pseudomonas sp.	?	-	?
additional information	substrate specificity, overview	Pseudomonas sp. CF 600	?	-	?

Subunits

Subunits	Comment	Organism
hexamer	subunits are arranged with 32 symmetry	Pseudomonas sp.
More	monomeric and dimeric structure comparison of 4-oxalocrotonate tautomerase with monomeric 5-carboxymethyl-2-hydroxymuconate isomerase, overview. The region of greatest similarity between the two enzymes is a large pocket that is proposed to be the active site	Pseudomonas sp.

Synonyms

Synonyms	Comment	Organism
4-OT	-	Pseudomonas sp.
4-oxalocrotonate tautomerase	-	Pseudomonas sp.

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Release 2023.1 (January 2023)