Literature summary for 5.3.2.5 extracted from

Carre-Mlouka, A.; Mejean, A.; Quillardet, P.; Ashida, H.; Saito, Y.; Yokota, A.; Callebaut, I.; Sekowska, A.; Dittmann, E.; Bouchier, C.; de Marsac, N.T.
A new rubisco-like protein coexists with a photosynthetic rubisco in the planktonic cyanobacteria Microcystis (2006), J. Biol. Chem., 281, 24462-24471.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene rbcLIV, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, cloning in Escherichia coli strain JM109, expression in Escherichia coli strain BL21	Microcystis aeruginosa

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Microcystis aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
5-methylthio-2,3-dioxo-pentyl phosphate	Microcystis aeruginosa	purified recombinant rubisco-like protein RbcLIV, overexpressed in Escherichia coli cells, does not display CO2 fixation activity but catalyzes enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate	2-hydroxy-5-methylthio-3-oxopentenyl phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Microcystis aeruginosa	-	planktonic organism	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-methylthio-2,3-dioxo-pentyl phosphate	purified recombinant rubisco-like protein RbcLIV, overexpressed in Escherichia coli cells, does not display CO2 fixation activity but catalyzes enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate	Microcystis aeruginosa	2-hydroxy-5-methylthio-3-oxopentenyl phosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	RbcLIV secondary structure analysis, spectroscopic analysis, and three-dimensional structure modeling, overview	Microcystis aeruginosa

Synonyms

Synonyms	Comment	Organism
RuBisCO-like protein	-	Microcystis aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Microcystis aeruginosa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Microcystis aeruginosa

General Information

General Information	Comment	Organism
evolution	members of the form IV subfamily, Rubisco-like proteins or RLPs, do not display Rubisco activity, but the two eubacterial members of this family play a role in sulfur metabolism. Discovery of the coexistence of RbcLI and RbcLIV in cyanobacteria, the ancestors of chloroplasts, enlightens episodes of the chaotic evolutionary history of the Rubiscos, a protein family of major importance for life on earth	Microcystis aeruginosa
additional information	the rbcLIV gene rescues a Bacillus subtilis MtnW-deficient mutant	Microcystis aeruginosa

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Release 2023.1 (January 2023)