Any feedback?
Literature summary for 5.3.2.5 extracted from
- Roles of RubisCO and the RubisCO-like protein in 5-methylthioadenosine metabolism in the nonsulfur purple bacterium Rhodospirillum rubrum (2010), J. Bacteriol., 192, 1324-1331.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene rlpA, form II RubisCO-like protein, cloning and expression in Escherichia coli strain DH5alpha | Rhodospirillum rubrum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| I19A | a form II RubisCO RLP disruption mutant strain | Rhodospirillum rubrum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-(methylthio)-2,3-dioxopentyl phosphate | Rhodospirillum rubrum | Rhodospirillum rubrum Form II RubisCO | 2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate | - |
? |  |
| 5-(methylthio)-2,3-dioxopentyl phosphate | Rhodospirillum rubrum Str-2 | Rhodospirillum rubrum Form II RubisCO | 2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate | - |
? | |
| 5-methylthioribulose 1-phosphate | Rhodospirillum rubrum | Rhodospirillum rubrum RLP | 1-thiomethyl-D-xylulose 5-phosphate + 1-thiomethyl-D-ribulose 5-phosphate | - |
ir | |
| 5-methylthioribulose 1-phosphate | Rhodospirillum rubrum Str-2 | Rhodospirillum rubrum RLP | 1-thiomethyl-D-xylulose 5-phosphate + 1-thiomethyl-D-ribulose 5-phosphate | - |
ir | |
| additional information | Rhodospirillum rubrum | the form II RubisCO enzyme from the nonsulfur purple bacterium Rhodospirillum rubrum is also able to function as an enolase in vivo as part of an methionine salvage pathway, but only under anaerobic conditions | ? | - |
? | |
| additional information | Rhodospirillum rubrum Str-2 | the form II RubisCO enzyme from the nonsulfur purple bacterium Rhodospirillum rubrum is also able to function as an enolase in vivo as part of an methionine salvage pathway, but only under anaerobic conditions | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodospirillum rubrum | - |
a spontaneous streptomycin-resistant derivative of strain S1, gene rlpA | - |
| Rhodospirillum rubrum Str-2 | - |
a spontaneous streptomycin-resistant derivative of strain S1, gene rlpA | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-(methylthio)-2,3-dioxopentyl phosphate | Rhodospirillum rubrum Form II RubisCO | Rhodospirillum rubrum | 2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate | - |
? | |
| 5-(methylthio)-2,3-dioxopentyl phosphate | Rhodospirillum rubrum Form II RubisCO | Rhodospirillum rubrum Str-2 | 2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate | - |
? | |
| 5-methylthioribulose 1-phosphate | Rhodospirillum rubrum RLP | Rhodospirillum rubrum | 1-thiomethyl-D-xylulose 5-phosphate + 1-thiomethyl-D-ribulose 5-phosphate | - |
ir | |
| 5-methylthioribulose 1-phosphate | Rhodospirillum rubrum RLP | Rhodospirillum rubrum Str-2 | 1-thiomethyl-D-xylulose 5-phosphate + 1-thiomethyl-D-ribulose 5-phosphate | - |
ir | |
| additional information | the form II RubisCO enzyme from the nonsulfur purple bacterium Rhodospirillum rubrum is also able to function as an enolase in vivo as part of an methionine salvage pathway, but only under anaerobic conditions | Rhodospirillum rubrum | ? | - |
? | |
| additional information | the form II RubisCO enzyme from the nonsulfur purple bacterium Rhodospirillum rubrum is also able to function as an enolase in vivo as part of an methionine salvage pathway, but only under anaerobic conditions | Rhodospirillum rubrum Str-2 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RLP | - |
Rhodospirillum rubrum |
| RuBisCO-like protein | - |
Rhodospirillum rubrum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the RubisCO-like protein, RLP, family of proteins. Unlike Bacillus subtilis RLP, Rodospirillum rubrum RLP does not catalyze the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate. Instead, under aerobic growth conditions, R. rubrum RLP employs another intermediate of the MSP, 5-methylthioribulose-1-phosphate, as a substrate, resulting in the formation of different products | Rhodospirillum rubrum |
| malfunction | the rlpA disruption strain of Rhodospirillum rubrum was incapable of using 5-methylthioadenosine as the sole sulfur source under aerobic growth conditions | Rhodospirillum rubrum |
| malfunction | the RLP disruption strain of Rhodospirillum rubrum is incapable of 5-methylthioadenosine-dependent growth under aerobic growth conditions. The rlpA disruption strain of Rhodospirillum rubrum is incapable of using 5-methylthioadenosine as the sole sulfur source under aerobic growth conditions | Rhodospirillum rubrum |
| physiological function | RLP is required for metabolizing 5-methylthioadenosine | Rhodospirillum rubrum |
| physiological function | RubisCO-like protein, RLP, is required for metabolizing 5-methylthioadenosine.the form II RubisCO enzyme from the nonsulfur purple bacterium Rhodospirillum rubrum is also able to function as an enolase in vivo as part of an methionine salvage pathway, but only under anaerobic conditions | Rhodospirillum rubrum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
