Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) pLysS cells | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
K122E | the mutant fails to form a catalytic dimer and does not show any DK-MTP-1-P enolase activity | Bacillus subtilis |
K122M | the mutant fails to form a catalytic dimer and does not show any DK-MTP-1-P enolase activity | Bacillus subtilis |
K122R | the mutant has specific activity of 32% less than that of the wild type enzyme and a lower thermal stability than the wild type enzyme | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
5-(methylthio)-2,3-dioxopentyl 1-phosphate | wild type enzyme, in 50 mM Tris-HCl (pH 8.2), 1 mM MgCl2, at 25°C | Bacillus subtilis | |
0.013 | - |
5-(methylthio)-2,3-dioxopentyl 1-phosphate | mutant enzyme K122R, in 50 mM Tris-HCl (pH 8.2), 1 mM MgCl2, at 25°C | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-Bind resin column chromatography and Superose 6 gel filtration | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-(methylthio)-2,3-dioxopentyl 1-phosphate | - |
Bacillus subtilis | 2-hydroxy-3-oxo-5-methylthiopentenyl 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
DK-MTP-1-P enolase | - |
Bacillus subtilis |
ribulose-1,5-bisphosphate carboxylase/oxygenase-like protein | - |
Bacillus subtilis |
RLP | - |
Bacillus subtilis |
RuBisCO-like protein | - |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
the wild type enzyme shows no decline in activity after incubation at 40°C, and retains about 80% of its original activity after incubation at 45°C | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
56 | - |
5-(methylthio)-2,3-dioxopentyl 1-phosphate | mutant enzyme K122R, in 50 mM Tris-HCl (pH 8.2), 1 mM MgCl2, at 25°C | Bacillus subtilis | |
83 | - |
5-(methylthio)-2,3-dioxopentyl 1-phosphate | wild type enzyme, in 50 mM Tris-HCl (pH 8.2), 1 mM MgCl2, at 25°C | Bacillus subtilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4400 | - |
5-(methylthio)-2,3-dioxopentyl 1-phosphate | mutant enzyme K122R, in 50 mM Tris-HCl (pH 8.2), 1 mM MgCl2, at 25°C | Bacillus subtilis | |
7100 | - |
5-(methylthio)-2,3-dioxopentyl 1-phosphate | wild type enzyme, in 50 mM Tris-HCl (pH 8.2), 1 mM MgCl2, at 25°C | Bacillus subtilis |