Cloned (Comment) | Organism |
---|---|
overexpression in Klebsiella pneumoniae strain CG253 leading to 5-6fold increased activity compared to the parent strain | Klebsiella pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by phosphate | Klebsiella pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25500 | - |
gel filtration | Klebsiella pneumoniae |
28300 | - |
1 * 28300, SDS-PAGE | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-diketo-1-phosphohexane | Klebsiella pneumoniae | an alternate substrate for the methionine salvage pathway | 2-hydroxy-3-keto-l-phospho-l-hexene | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Klebsiella pneumoniae CG253 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme by fractionation with help of protamine suzlfate, ammonium sulfate, and P-60, followed by anion exchange and hydrophobic interaction chromatography | Klebsiella pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
overall pathway activity, purified enzyme, pH 7.5, 30°C | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-diketo-1-phosphohexane | an alternate substrate for the methionine salvage pathway | Klebsiella pneumoniae | 2-hydroxy-3-keto-l-phospho-l-hexene | - |
? | |
2,3-diketo-1-phosphohexane | an analogue to 2,3-diketo-5-methylthio-1-phosphopentane | Klebsiella pneumoniae | 2-hydroxy-3-keto-l-phospho-l-hexene | a chromophoric intermediate compound, product determination by NMR analysis | ? | |
2,3-diketo-l-phospho-5-thiomethylpentane | - |
Klebsiella pneumoniae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 28300, SDS-PAGE | Klebsiella pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Klebsiella pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Klebsiella pneumoniae |
General Information | Comment | Organism |
---|---|---|
metabolism | Klebsiella pneumoniae converts 2,3-diketo-5-methylthio-l-phosphopentane to formate, 2-keto-4-methylthiobutyrate, and 3-methylthiopropionate. 2,3-diketo-1-phosphohexane, the desthio analogue of the naturally occurring diketone, can serve as alternate substrate in the methionine salvage pathway | Klebsiella pneumoniae |