Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces fradiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose | pH 7.5, 25°C | Streptomyces fradiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18806 | - |
2 * 18806, calculated from sequence | Streptomyces fradiae |
19000 | - |
2 * 19000, SDS-PAGE | Streptomyces fradiae |
31000 | - |
gel filtration | Streptomyces fradiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-4-dehydro-6-deoxy-alpha-D-glucose | Streptomyces fradiae | the enzyme is involved in the D-mycaminose biosynthetic pathway | dTDP-3-dehydro-6-deoxy-alpha-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces fradiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces fradiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP-4-dehydro-6-deoxy-alpha-D-glucose | efficiency of conversion is low, requiring a high concentration of enzyme and a long incubation time to achieve significant turnover | Streptomyces fradiae | CDP-3-dehydro-6-deoxy-alpha-D-glucose | - |
? | |
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose | efficiency is comparable to that with dTDP-6-deoxy-D-xylo-hexopyranos-4-ulose | Streptomyces fradiae | dTDP-3-dehydro-2,6-dideoxy-alpha-D-glucose | - |
? | |
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose | - |
Streptomyces fradiae | dTDP-3-dehydro-6-deoxy-alpha-D-glucopyranose | - |
? | |
dTDP-4-dehydro-6-deoxy-alpha-D-glucose | the enzyme is involved in the D-mycaminose biosynthetic pathway | Streptomyces fradiae | dTDP-3-dehydro-6-deoxy-alpha-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 19000, SDS-PAGE | Streptomyces fradiae |
dimer | 2 * 18806, calculated from sequence | Streptomyces fradiae |
Synonyms | Comment | Organism |
---|---|---|
TDP-4-keto-6-deoxy-D-glucose-3,4-ketoisomerase | - |
Streptomyces fradiae |
Tyl1a | - |
Streptomyces fradiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Streptomyces fradiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00025 | - |
CDP-4-dehydro-6-deoxy-alpha-D-glucose | pH 7.5, 25°C | Streptomyces fradiae | |
0.1 | - |
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose | pH 7.5, 25°C | Streptomyces fradiae | |
0.13 | - |
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose | pH 7.5, 25°C | Streptomyces fradiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay | Streptomyces fradiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.7 | - |
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose | pH 7.5, 25°C | Streptomyces fradiae |