General Stability | Organism |
---|---|
oxaloacetate tautomerase-1 resists freezing and thawing when dissolved in potassium phosphate buffer, pH 7.8 | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | enzyme form OAT-2 is inhibited, enzyme form OAT-1 not | Bos taurus | |
Maleate | - |
Bos taurus | |
malonate | enzyme form OAT-2 is inhibited, enzyme form OAT-1 not | Bos taurus | |
oxalate | - |
Bos taurus | |
Oxaloacetic acid diethylester | enzyme form OAT-1 is inhibited, enzyme form OAT-2 not | Bos taurus | |
phenylpyruvate | enzyme form OAT-2 is inhibited, enzyme form OAT-1 not | Bos taurus | |
phosphoenolpyruvate | enzyme form OAT-2 is inhibited, enzyme form OAT-1 not | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
enol-oxaloacetate | enzyme form OAT-1 | Bos taurus | |
0.068 | - |
keto-oxaloacetate | enzyme form OAT-1 | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial matrix | about 30% of the tautomerase activity in the matrix are represented by oxaloacetate tautomerase-1 and about 70% by oxaloacetate tautomerase-2 | Bos taurus | 5759 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
enzyme form OAT-1, gel filtration | Bos taurus |
80000 | - |
enzyme form OAT-2, gel filtration | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
enzyme form OAT-1 and enzyme form OAT-2 | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Bos taurus | - |
Storage Stability | Organism |
---|---|
0°C, oxaloacetate tautomerase-1 is quite stable for several days | Bos taurus |
0°C, oxaloacetate tautomerase-2 gradually loses activity within several days | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
keto-Oxaloacetate | r | Bos taurus | Enol-oxaloacetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 37000, enzyme form OAT-1, SDS-PAGE | Bos taurus |
monomer | 1 * 80000, enzyme form OAT-2, SDS-PAGE | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26.7 | - |
enol-oxaloacetate | enzyme form OAT-2, 25°C, pH 9.0 | Bos taurus | |
45.7 | - |
enol-oxaloacetate | enzyme form OAT-1, 25°C, pH 9.0 | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 10 | oxaloacetate tautomerase-1 | Bos taurus |
9 | - |
oxaloacetate tautomerase-2 | Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | pH 7.0: about 50% of maximal activity, pH 8.5-10.0: maximal activity, oxaloacetate tautomerase-1 | Bos taurus |
7.7 | 9.5 | pH 7.7: about 20% of maximal activity, pH 9.5: about 40% of maximal activity, oxaloacetate tautomerase-2 | Bos taurus |