Literature summary for 5.3.2.1 extracted from

Taylor, A.B.; Johnson, W.H., Jr.; Czerwinski, R.M.; Li, H.S.; Hackert, M.L.; Whitman, C.P.
Crystal structure of macrophage migration inhibitory factor complexed with (E)-2-fluoro-p-hydroxycinnamate at 1.8 A resolution: implications for enzymatic catalysis and inhibition (1999), Biochemistry, 38, 7444-7452.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure of a complex between enzyme and the competitive inhibitor (E)-2-fluoro-o-hydroxycinnamate determined to 1.8 A resolution	Mus musculus

Inhibitors

Inhibitors	Comment	Organism	Structure
(E)-2-fluoro-o-hydroxycinnamate	competitive	Mus musculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.13	-	enol-phenylpyruvate	23°C, pH 6.5	Mus musculus
0.17	-	enol-(p-hydroxyphenyl)pyruvate	23°C, pH 6.5	Mus musculus
2.77	-	keto-(p-hydroxyphenyl)pyruvate	23°C, pH 6.5	Mus musculus
4.9	-	keto-phenylpyruvate	23°C, pH 6.5	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	P34884	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
keto-(p-hydroxyphenyl)pyruvate	-	Mus musculus	enol-(p-hydroxyphenyl)pyruvate	-	r
keto-phenylpyruvate	-	Mus musculus	enol-phenylpyruvate	-	r

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
120	-	keto-(p-hydroxyphenyl)pyruvate	23°C, pH 6.5	Mus musculus
160	-	enol-(p-hydroxyphenyl)pyruvate	23°C, pH 6.5	Mus musculus
285	-	keto-phenylpyruvate	23°C, pH 6.5	Mus musculus
290	-	enol-phenylpyruvate	23°C, pH 6.5	Mus musculus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0026	-	(E)-2-fluoro-o-hydroxycinnamate	23°C, pH 6.5	Mus musculus

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Release 2023.1 (January 2023)