Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.1.B3 extracted from

  • Morimoto, K.; Terami, Y.; Maeda, Y.; Yoshihara, A.; Takata, G.; Izumori, K.
    Cloning and characterization of the L-ribose isomerase gene from Cellulomonas parahominis MB426 (2013), J. Biosci. Bioeng., 115, 377-381.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Cellulomonas parahominis

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Cellulomonas parahominis
Ba2+
-
Cellulomonas parahominis
Ca2+
-
Cellulomonas parahominis
Cu2+
-
Cellulomonas parahominis
Fe2+
-
Cellulomonas parahominis
Li+
-
Cellulomonas parahominis
Ni2+
-
Cellulomonas parahominis
Zn2+
-
Cellulomonas parahominis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
15.4
-
L-ribulose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
30.4
-
L-ribose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
32.8
-
D-xylulose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
37.1
-
D-Lyxose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
37.6
-
D-talose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
39.2
-
D-tagatose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
40
-
L-sorbose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
40.1
-
L-gulose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
40.3
-
L-allose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
51
-
D-fructose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
51.1
-
D-mannose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
54.8
-
L-psicose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme is not dependent for metallic ions for its activity Cellulomonas parahominis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27435
-
x * 27435, calculated from amino acid sequence Cellulomonas parahominis
32000
-
x * 32000, SDS-PAGE Cellulomonas parahominis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-ribose Cellulomonas parahominis highest activity with L-ribose L-ribulose
-
r
L-ribose Cellulomonas parahominis MB426 highest activity with L-ribose L-ribulose
-
r
L-ribulose Cellulomonas parahominis
-
L-ribose
-
r

Organism

Organism UniProt Comment Textmining
Cellulomonas parahominis L0N3Y0
-
-
Cellulomonas parahominis MB426 L0N3Y0
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Q Sepharose column chromatography, Resource PHE column chromatographym and Resource Q column chromatography Cellulomonas parahominis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.49
-
crude native enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
4.9
-
crude recombinant enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
39.5
-
native enzyme after 27fold purification, in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
214
-
recombinant enzyme after 43.6fold purification, in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose
-
Cellulomonas parahominis D-mannose
-
r
D-Lyxose 43.4% activity compared to L-ribose Cellulomonas parahominis D-Xylulose
-
r
D-Lyxose 43.4% activity compared to L-ribose Cellulomonas parahominis MB426 D-Xylulose
-
r
D-Mannose 2.07% activity compared to L-ribose Cellulomonas parahominis D-Fructose
-
r
D-Mannose 2.07% activity compared to L-ribose Cellulomonas parahominis MB426 D-Fructose
-
r
D-tagatose
-
Cellulomonas parahominis D-talose
-
r
D-talose 36.8% activity compared to L-ribose Cellulomonas parahominis D-tagatose
-
r
D-talose 36.8% activity compared to L-ribose Cellulomonas parahominis MB426 D-tagatose
-
r
D-xylulose
-
Cellulomonas parahominis D-lyxose
-
r
L-allose 8.34% activity compared to L-ribose Cellulomonas parahominis L-psicose
-
r
L-allose 8.34% activity compared to L-ribose Cellulomonas parahominis MB426 L-psicose
-
r
L-gulose 2.27% activity compared to L-ribose Cellulomonas parahominis L-sorbose
-
r
L-psicose
-
Cellulomonas parahominis L-allose
-
r
L-ribose highest activity with L-ribose Cellulomonas parahominis L-ribulose
-
r
L-ribose highest activity with L-ribose Cellulomonas parahominis MB426 L-ribulose
-
r
L-ribulose
-
Cellulomonas parahominis L-ribose
-
r
L-sorbose
-
Cellulomonas parahominis L-gulose
-
r

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Cellulomonas parahominis
? x * 27435, calculated from amino acid sequence Cellulomonas parahominis

Synonyms

Synonyms Comment Organism
LRI
-
Cellulomonas parahominis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Cellulomonas parahominis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
the enzyme is stable up to 40°C for 1 h Cellulomonas parahominis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.38
-
L-sorbose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
2.1
-
L-gulose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
2.2
-
L-psicose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
2.5
-
D-mannose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
2.8
-
L-allose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
15.8
-
D-talose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
17.3
-
D-Lyxose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
17.5
-
D-fructose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
18.2
-
D-xylulose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
40.8
-
D-tagatose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
95.2
-
L-ribose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
108.8
-
L-ribulose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Cellulomonas parahominis

pH Range

pH Minimum pH Maximum Comment Organism
6 11 more than 60% activity between pH 6.0 and 11.0 Cellulomonas parahominis

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 10 the enzyme is stable in a pH range of pH 7.0-10.0 at 4°C for 24 h in any buffer Cellulomonas parahominis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.009
-
L-sorbose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
0.034
-
D-fructose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
0.04
-
L-psicose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
0.048
-
D-mannose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
0.054
-
L-gulose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
0.07
-
L-allose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
0.104
-
D-tagatose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
0.42
-
D-talose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
0.47
-
D-Lyxose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
0.56
-
D-xylulose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
3.13
-
L-ribose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
7.05
-
L-ribulose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis