Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Cellulomonas parahominis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Cellulomonas parahominis | |
Ba2+ | - |
Cellulomonas parahominis | |
Ca2+ | - |
Cellulomonas parahominis | |
Cu2+ | - |
Cellulomonas parahominis | |
Fe2+ | - |
Cellulomonas parahominis | |
Li+ | - |
Cellulomonas parahominis | |
Ni2+ | - |
Cellulomonas parahominis | |
Zn2+ | - |
Cellulomonas parahominis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.4 | - |
L-ribulose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
30.4 | - |
L-ribose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
32.8 | - |
D-xylulose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
37.1 | - |
D-Lyxose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
37.6 | - |
D-talose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
39.2 | - |
D-tagatose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
40 | - |
L-sorbose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
40.1 | - |
L-gulose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
40.3 | - |
L-allose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
51 | - |
D-fructose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
51.1 | - |
D-mannose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
54.8 | - |
L-psicose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not dependent for metallic ions for its activity | Cellulomonas parahominis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27435 | - |
x * 27435, calculated from amino acid sequence | Cellulomonas parahominis |
32000 | - |
x * 32000, SDS-PAGE | Cellulomonas parahominis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ribose | Cellulomonas parahominis | highest activity with L-ribose | L-ribulose | - |
r | |
L-ribose | Cellulomonas parahominis MB426 | highest activity with L-ribose | L-ribulose | - |
r | |
L-ribulose | Cellulomonas parahominis | - |
L-ribose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellulomonas parahominis | L0N3Y0 | - |
- |
Cellulomonas parahominis MB426 | L0N3Y0 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Q Sepharose column chromatography, Resource PHE column chromatographym and Resource Q column chromatography | Cellulomonas parahominis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.49 | - |
crude native enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis |
4.9 | - |
crude recombinant enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis |
39.5 | - |
native enzyme after 27fold purification, in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis |
214 | - |
recombinant enzyme after 43.6fold purification, in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose | - |
Cellulomonas parahominis | D-mannose | - |
r | |
D-Lyxose | 43.4% activity compared to L-ribose | Cellulomonas parahominis | D-Xylulose | - |
r | |
D-Lyxose | 43.4% activity compared to L-ribose | Cellulomonas parahominis MB426 | D-Xylulose | - |
r | |
D-Mannose | 2.07% activity compared to L-ribose | Cellulomonas parahominis | D-Fructose | - |
r | |
D-Mannose | 2.07% activity compared to L-ribose | Cellulomonas parahominis MB426 | D-Fructose | - |
r | |
D-tagatose | - |
Cellulomonas parahominis | D-talose | - |
r | |
D-talose | 36.8% activity compared to L-ribose | Cellulomonas parahominis | D-tagatose | - |
r | |
D-talose | 36.8% activity compared to L-ribose | Cellulomonas parahominis MB426 | D-tagatose | - |
r | |
D-xylulose | - |
Cellulomonas parahominis | D-lyxose | - |
r | |
L-allose | 8.34% activity compared to L-ribose | Cellulomonas parahominis | L-psicose | - |
r | |
L-allose | 8.34% activity compared to L-ribose | Cellulomonas parahominis MB426 | L-psicose | - |
r | |
L-gulose | 2.27% activity compared to L-ribose | Cellulomonas parahominis | L-sorbose | - |
r | |
L-psicose | - |
Cellulomonas parahominis | L-allose | - |
r | |
L-ribose | highest activity with L-ribose | Cellulomonas parahominis | L-ribulose | - |
r | |
L-ribose | highest activity with L-ribose | Cellulomonas parahominis MB426 | L-ribulose | - |
r | |
L-ribulose | - |
Cellulomonas parahominis | L-ribose | - |
r | |
L-sorbose | - |
Cellulomonas parahominis | L-gulose | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE | Cellulomonas parahominis |
? | x * 27435, calculated from amino acid sequence | Cellulomonas parahominis |
Synonyms | Comment | Organism |
---|---|---|
LRI | - |
Cellulomonas parahominis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Cellulomonas parahominis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
the enzyme is stable up to 40°C for 1 h | Cellulomonas parahominis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
L-sorbose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
2.1 | - |
L-gulose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
2.2 | - |
L-psicose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
2.5 | - |
D-mannose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
2.8 | - |
L-allose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
15.8 | - |
D-talose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
17.3 | - |
D-Lyxose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
17.5 | - |
D-fructose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
18.2 | - |
D-xylulose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
40.8 | - |
D-tagatose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
95.2 | - |
L-ribose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
108.8 | - |
L-ribulose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Cellulomonas parahominis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | more than 60% activity between pH 6.0 and 11.0 | Cellulomonas parahominis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | the enzyme is stable in a pH range of pH 7.0-10.0 at 4°C for 24 h in any buffer | Cellulomonas parahominis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
L-sorbose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
0.034 | - |
D-fructose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
0.04 | - |
L-psicose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
0.048 | - |
D-mannose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
0.054 | - |
L-gulose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
0.07 | - |
L-allose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
0.104 | - |
D-tagatose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
0.42 | - |
D-talose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
0.47 | - |
D-Lyxose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
0.56 | - |
D-xylulose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
3.13 | - |
L-ribose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis | |
7.05 | - |
L-ribulose | in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C | Cellulomonas parahominis |