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Literature summary for 5.3.1.9 extracted from

  • Myung, S.; Zhang, X.Z.; Percival Zhang, Y.H.
    Ultra-stable phosphoglucose isomerase through immobilization of cellulose-binding module-tagged thermophilic enzyme on low-cost high-capacity cellulosic adsorbent (2011), Biotechnol. Prog., 27, 969-975.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star (DE3) cells Acetivibrio thermocellus

General Stability

General Stability Organism
phosphoglucose isomerase in Tris-HCl buffer has 30% lower observed activity than that in the HEPES buffer at pH 7.0. At working conditions (100 mM HEPES buffer (pH 7.5) containing 10 mM Mg2+ and 0.5 mM Mn2+ at 60°C), no significant activity is lost after 50 washings of immobilized cellulose-binding module-phosphoglucose isomerase Acetivibrio thermocellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.02
-
D-glucose 6-phosphate cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C Acetivibrio thermocellus
1.53
-
D-glucose 6-phosphate avicel-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C Acetivibrio thermocellus
1.65
-
D-glucose 6-phosphate avicel-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C Acetivibrio thermocellus
1.86
-
D-glucose 6-phosphate cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C Acetivibrio thermocellus
1.9
-
D-glucose 6-phosphate free enzyme, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C Acetivibrio thermocellus
2.11
-
D-glucose 6-phosphate immobilized-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C Acetivibrio thermocellus
2.43
-
D-glucose 6-phosphate immobilized-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C Acetivibrio thermocellus
2.89
-
D-glucose 6-phosphate free enzyme, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C Acetivibrio thermocellus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
x * 49000, free enzyme, SDS-PAGE Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity adsorption on regenerated amorphous cellulose and intein cleavage or ethylene glycol elusion method Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate
-
Acetivibrio thermocellus D-fructose 6-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 49000, free enzyme, SDS-PAGE Acetivibrio thermocellus

Synonyms

Synonyms Comment Organism
Phosphoglucose isomerase
-
Acetivibrio thermocellus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
free and immobilized enzyme, at pH 7.5 Acetivibrio thermocellus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 70 free and immobilized enzymes retain about 90% of their maximum activity at 50°C and 70°C, but only about 40% of optimum activity is exhibited at 30°C Acetivibrio thermocellus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
free phosphoglucose isomerase is stable at a high protein concentration of 0.1 g/l (half-life of 180 h at 60°C) but deactivates rapidly at low concentrations (half-life at 60°C is 2.4 h at 0.001 g/l). Immobilized cellulose-binding module-phosphoglucose isomerase at a low concentration of 0.001 g/l has a half-life of 190 h, approximately 80fold of that of free phosphoglucose isomerase. Immobilized cellulose-binding module-phosphoglucose isomerase on regenerated amorphous cellulose is extremely stable at about 60°C, nearly independent of its mass concentration in bulk solution Acetivibrio thermocellus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
729
-
D-glucose 6-phosphate cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C Acetivibrio thermocellus
929
-
D-glucose 6-phosphate free enzyme, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C Acetivibrio thermocellus
946
-
D-glucose 6-phosphate avicel-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C Acetivibrio thermocellus
1091
-
D-glucose 6-phosphate immobilized-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C Acetivibrio thermocellus
2009
-
D-glucose 6-phosphate avicel-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C Acetivibrio thermocellus
2198
-
D-glucose 6-phosphate immobilized-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C Acetivibrio thermocellus
2433
-
D-glucose 6-phosphate cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C Acetivibrio thermocellus
2765
-
D-glucose 6-phosphate free enzyme, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
free and immobilized enzyme, in HEPES and TAPS buffers Acetivibrio thermocellus

pH Range

pH Minimum pH Maximum Comment Organism
7 9 free and immobilized enzymes have approximately 75% and 40% of their maximum activity at pH 7.0 and pH 9.0 Acetivibrio thermocellus

General Information

General Information Comment Organism
metabolism phosphoglucose isomerase is a key enzyme in the glycolysis and glycogenesis pathways Acetivibrio thermocellus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
489
-
D-glucose 6-phosphate free enzyme, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C Acetivibrio thermocellus
517
-
D-glucose 6-phosphate immobilized-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C Acetivibrio thermocellus
618
-
D-glucose 6-phosphate avicel-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C Acetivibrio thermocellus
715
-
D-glucose 6-phosphate cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C Acetivibrio thermocellus
906
-
D-glucose 6-phosphate immobilized-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C Acetivibrio thermocellus
957
-
D-glucose 6-phosphate free enzyme, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C Acetivibrio thermocellus
1218
-
D-glucose 6-phosphate avicel-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C Acetivibrio thermocellus
1308
-
D-glucose 6-phosphate cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C Acetivibrio thermocellus