Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star (DE3) cells | Acetivibrio thermocellus |
General Stability | Organism |
---|---|
phosphoglucose isomerase in Tris-HCl buffer has 30% lower observed activity than that in the HEPES buffer at pH 7.0. At working conditions (100 mM HEPES buffer (pH 7.5) containing 10 mM Mg2+ and 0.5 mM Mn2+ at 60°C), no significant activity is lost after 50 washings of immobilized cellulose-binding module-phosphoglucose isomerase | Acetivibrio thermocellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.02 | - |
D-glucose 6-phosphate | cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C | Acetivibrio thermocellus | |
1.53 | - |
D-glucose 6-phosphate | avicel-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C | Acetivibrio thermocellus | |
1.65 | - |
D-glucose 6-phosphate | avicel-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C | Acetivibrio thermocellus | |
1.86 | - |
D-glucose 6-phosphate | cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C | Acetivibrio thermocellus | |
1.9 | - |
D-glucose 6-phosphate | free enzyme, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C | Acetivibrio thermocellus | |
2.11 | - |
D-glucose 6-phosphate | immobilized-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C | Acetivibrio thermocellus | |
2.43 | - |
D-glucose 6-phosphate | immobilized-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C | Acetivibrio thermocellus | |
2.89 | - |
D-glucose 6-phosphate | free enzyme, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C | Acetivibrio thermocellus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
x * 49000, free enzyme, SDS-PAGE | Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity adsorption on regenerated amorphous cellulose and intein cleavage or ethylene glycol elusion method | Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate | - |
Acetivibrio thermocellus | D-fructose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49000, free enzyme, SDS-PAGE | Acetivibrio thermocellus |
Synonyms | Comment | Organism |
---|---|---|
Phosphoglucose isomerase | - |
Acetivibrio thermocellus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
free and immobilized enzyme, at pH 7.5 | Acetivibrio thermocellus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | free and immobilized enzymes retain about 90% of their maximum activity at 50°C and 70°C, but only about 40% of optimum activity is exhibited at 30°C | Acetivibrio thermocellus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
free phosphoglucose isomerase is stable at a high protein concentration of 0.1 g/l (half-life of 180 h at 60°C) but deactivates rapidly at low concentrations (half-life at 60°C is 2.4 h at 0.001 g/l). Immobilized cellulose-binding module-phosphoglucose isomerase at a low concentration of 0.001 g/l has a half-life of 190 h, approximately 80fold of that of free phosphoglucose isomerase. Immobilized cellulose-binding module-phosphoglucose isomerase on regenerated amorphous cellulose is extremely stable at about 60°C, nearly independent of its mass concentration in bulk solution | Acetivibrio thermocellus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
729 | - |
D-glucose 6-phosphate | cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C | Acetivibrio thermocellus | |
929 | - |
D-glucose 6-phosphate | free enzyme, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C | Acetivibrio thermocellus | |
946 | - |
D-glucose 6-phosphate | avicel-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C | Acetivibrio thermocellus | |
1091 | - |
D-glucose 6-phosphate | immobilized-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C | Acetivibrio thermocellus | |
2009 | - |
D-glucose 6-phosphate | avicel-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C | Acetivibrio thermocellus | |
2198 | - |
D-glucose 6-phosphate | immobilized-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C | Acetivibrio thermocellus | |
2433 | - |
D-glucose 6-phosphate | cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C | Acetivibrio thermocellus | |
2765 | - |
D-glucose 6-phosphate | free enzyme, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C | Acetivibrio thermocellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
free and immobilized enzyme, in HEPES and TAPS buffers | Acetivibrio thermocellus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | free and immobilized enzymes have approximately 75% and 40% of their maximum activity at pH 7.0 and pH 9.0 | Acetivibrio thermocellus |
General Information | Comment | Organism |
---|---|---|
metabolism | phosphoglucose isomerase is a key enzyme in the glycolysis and glycogenesis pathways | Acetivibrio thermocellus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
489 | - |
D-glucose 6-phosphate | free enzyme, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C | Acetivibrio thermocellus | |
517 | - |
D-glucose 6-phosphate | immobilized-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C | Acetivibrio thermocellus | |
618 | - |
D-glucose 6-phosphate | avicel-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C | Acetivibrio thermocellus | |
715 | - |
D-glucose 6-phosphate | cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C | Acetivibrio thermocellus | |
906 | - |
D-glucose 6-phosphate | immobilized-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C | Acetivibrio thermocellus | |
957 | - |
D-glucose 6-phosphate | free enzyme, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C | Acetivibrio thermocellus | |
1218 | - |
D-glucose 6-phosphate | avicel-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C | Acetivibrio thermocellus | |
1308 | - |
D-glucose 6-phosphate | cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C | Acetivibrio thermocellus |