Literature summary for 5.3.1.9 extracted from

Sun, L.C.; Zhou, L.G.; Du, C.H.; Cai, Q.F.; Hara, K.; Su, W.J.; Cao, M.J.
Glucose-6-phosphate isomerase is an endogenous inhibitor to myofibril-bound serine proteinase of crucian carp (Carassius auratus) (2009), J. Agric. Food Chem., 57, 5549-5555.

Search for more literature for 5.3.1.9

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.36	-	D-fructose 6-phosphate	pH 8.0	Carassius auratus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	activates	Carassius auratus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55000	-	1 * 55000 + 1 * 65000, SDS-PAGE	Carassius auratus
65000	-	1 * 55000 + 1 * 65000, SDS-PAGE	Carassius auratus
120000	-	gel filtration	Carassius auratus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glucose 6-phosphate	Carassius auratus	-	D-fructose 6-phosphate	-	r
additional information	Carassius auratus	the multifunctional protein GPI shows specific and competitive inhibitory activity toward a myofibril-bound serine proteinase, MBSP, from Carassius auratus with a Ki of 320 nM, inhibition kinetics, while no inhibitory activity is identified toward other serine proteinases, such as white croaker MBSP and crucian carp trypsin, overview	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Carassius auratus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme 468fold from skeletal muscle to homogeneity by ammonium sulfate fractionation, anion exchange and cation exchange chromatography, followed by gel filtration	Carassius auratus

Source Tissue

Source Tissue	Comment	Organism	Textmining
skeletal muscle	-	Carassius auratus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
140	-	purified enzyme, substrate fructose 6-phosphate, reverse reaction	Carassius auratus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 6-phosphate	-	Carassius auratus	D-glucose 6-phosphate	-	r
D-glucose 6-phosphate	-	Carassius auratus	D-fructose 6-phosphate	-	r
additional information	the multifunctional protein GPI shows specific and competitive inhibitory activity toward a myofibril-bound serine proteinase, MBSP, from Carassius auratus with a Ki of 320 nM, inhibition kinetics, while no inhibitory activity is identified toward other serine proteinases, such as white croaker MBSP and crucian carp trypsin, overview	Carassius auratus	?	-	?

Subunits

Subunits	Comment	Organism
heterodimer	1 * 55000 + 1 * 65000, SDS-PAGE	Carassius auratus

Synonyms

Synonyms	Comment	Organism
PGI	-	Carassius auratus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
18	-	D-fructose 6-phosphate	pH 8.0	Carassius auratus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at, reverse reaction	Carassius auratus

General Information

General Information	Comment	Organism
physiological function	multifunctional protein GPI is an endogenous inhibitor to myofibril-bound serine proteinase, and may play a significant role in the regulation of muscular protein metabolism in vivo	Carassius auratus

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Release 2023.1 (January 2023)