Literature summary for 5.3.1.9 extracted from

Lin, H.; Kao, Y.; Chen, S.; Meng, M.
Effects of inherited mutations on catalytic activity and structural stability of human glucose-6-phosphate isomerase expressed in Escherichia coli (2009), Biochim. Biophys. Acta, 1794, 315-323.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and mutant enzymes in Escherichia coli strain DF2145	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
A300P	the mutation may affect the folding efficiency of the enzyme protein, the mutant shows reduced expression level and barely detectable activity	Homo sapiens
E495K	the mutation may affect the folding efficiency of the enzyme protein, the mutant shows reduced expression level and barely detectable activity	Homo sapiens
E495K	the mutation weakens network bonding of the enzyme	Homo sapiens
H389R	the mutation at or near the active site highly affects the catalytic efficiency of the enzyme, the mutant shows barely detectable activity	Homo sapiens
I525T	the mutation decreases the enzyme tolerance to heat or SDS by mechanisms of decreasing packing efficiency	Homo sapiens
I525T	the mutation destabilizes the ternary structure of the enzyme	Homo sapiens
L339P	the mutation decreases the enzyme tolerance to heat or SDS by mechanisms of decreasing packing efficiency	Homo sapiens
L339P	the mutation may affect the folding efficiency of the enzyme protein, the mutant shows reduced expression level and barely detectable activity	Homo sapiens
L487F	the mutation decreases the enzyme tolerance to heat or SDS by mechanisms of decreasing packing efficiency	Homo sapiens
additional information	thermoinactivation of GPI genetic variants, phenotypes, overview	Homo sapiens
R273H	the mutation at or near the active site highly affects the catalytic efficiency of the enzyme, the mutant shows barely detectable activity	Homo sapiens
R347C	the mutation destabilizes the ternary structure of the enzyme	Homo sapiens
R347C	the mutation weakens network bonding of the enzyme	Homo sapiens
R347H	the mutation destabilizes the ternary structure of the enzyme	Homo sapiens
R347H	the mutation weakens network bonding of the enzyme	Homo sapiens
R472H	the mutation weakens network bonding of the enzyme	Homo sapiens
R75G	the mutation weakens network bonding of the enzyme	Homo sapiens
R83W	the mutation increases the water-accessible hydrophobic surface	Homo sapiens
S278L	the mutation at or near the active site highly affects the catalytic efficiency of the enzyme	Homo sapiens
S278L	the mutation decreases the enzyme tolerance to heat or SDS by mechanisms of decreasing packing efficiency	Homo sapiens
T195I	the mutation decreases the enzyme tolerance to heat or SDS by mechanisms of decreasing packing efficiency	Homo sapiens
T195I	the mutation destabilizes the ternary structure of the enzyme	Homo sapiens
T375R	the mutation decreases the enzyme tolerance to heat or SDS by mechanisms of decreasing packing efficiency	Homo sapiens
V101M	the mutation decreases the enzyme tolerance to heat or SDS by mechanisms of decreasing packing efficiency	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.031	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant S278L	Homo sapiens
0.034	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant I525T	Homo sapiens
0.037	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant wild-type enzyme	Homo sapiens
0.038	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant R347H	Homo sapiens
0.038	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant R75G	Homo sapiens
0.039	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant L487F	Homo sapiens
0.045	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant A300P	Homo sapiens
0.045	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant L339P	Homo sapiens
0.046	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant R347C	Homo sapiens
0.046	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant T375R	Homo sapiens
0.05	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant E495K	Homo sapiens
0.061	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant R83W	Homo sapiens
0.063	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant T195I	Homo sapiens
0.063	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant V101M	Homo sapiens
0.068	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant R472H	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
D-fructose 6-phosphate	Homo sapiens	-	D-glucose 6-phosphate	-	r
D-glucose 6-phosphate	Homo sapiens	-	D-fructose 6-phosphate	-	r
additional information	Homo sapiens	the enzyme can also act as an autocrine motility factor, neuroleukin, and maturation factor	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P06744	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
erythrocyte	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
620	-	purified wild-type enzyme	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
D-fructose 6-phosphate	-	Homo sapiens	D-glucose 6-phosphate	-	r
D-glucose 6-phosphate	-	Homo sapiens	D-fructose 6-phosphate	-	r
additional information	the enzyme can also act as an autocrine motility factor, neuroleukin, and maturation factor	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
homodimer	-	Homo sapiens

Synonyms

Synonyms	Comment	Organism
GPI	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	thermoinactivation of GPI genetic variants, overview	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.3	-	D-glucose 6-phosphate	pH 7.5, 30°C, recombinant mutant S278L	Homo sapiens
0.6	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant S278L	Homo sapiens
15	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant L339P	Homo sapiens
17	-	D-glucose 6-phosphate	pH 7.5, 30°C, recombinant mutant L487F	Homo sapiens
19	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant L487F	Homo sapiens
31	-	D-glucose 6-phosphate	pH 7.5, 30°C, recombinant mutant L339P	Homo sapiens
42	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant R347H	Homo sapiens
61	-	D-glucose 6-phosphate	pH 7.5, 30°C, recombinant mutant A300P	Homo sapiens
63	-	D-glucose 6-phosphate	pH 7.5, 30°C, recombinant mutant R347H	Homo sapiens
75	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant T375R	Homo sapiens
76	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant I525T	Homo sapiens
80	-	D-glucose 6-phosphate	pH 7.5, 30°C, recombinant mutant T375R	Homo sapiens
93	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant R75G	Homo sapiens
100	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant A300P	Homo sapiens
110	-	D-glucose 6-phosphate	pH 7.5, 30°C, recombinant mutant I525T	Homo sapiens
120	-	D-glucose 6-phosphate	pH 7.5, 30°C, recombinant mutant R83W	Homo sapiens
130	-	D-glucose 6-phosphate	pH 7.5, 30°C, recombinant mutant R75G	Homo sapiens
140	-	D-glucose 6-phosphate	pH 7.5, 30°C, recombinant mutant V101M	Homo sapiens
150	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant R347C	Homo sapiens
160	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant V101M	Homo sapiens
190	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant R472H	Homo sapiens
190	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant R83W	Homo sapiens
260	-	D-glucose 6-phosphate	pH 7.5, 30°C, recombinant mutant R472H	Homo sapiens
300	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant T195I	Homo sapiens
350	-	D-glucose 6-phosphate	pH 7.5, 30°C, recombinant mutant R347C	Homo sapiens
380	-	D-glucose 6-phosphate	pH 7.5, 30°C, recombinant mutant T195I	Homo sapiens
420	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant mutant E495K	Homo sapiens
650	-	D-fructose 6-phosphate	pH 7.5, 30°C, recombinant wild-type enzyme	Homo sapiens
750	-	D-glucose 6-phosphate	pH 7.5, 30°C, recombinant mutant E495K	Homo sapiens
1000	-	D-glucose 6-phosphate	pH 7.5, 30°C, recombinant wild-type enzyme	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Homo sapiens

General Information

General Information	Comment	Organism
malfunction	deficiency of the enzymatic activity in red blood cells causes nonspherocytic hemolytic anemia	Homo sapiens