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Literature summary for 5.3.1.9 extracted from

  • Mathur, D.; Garg, L.C.
    Functional phosphoglucose isomerase from Mycobacterium tuberculosis H37Rv: rapid purification with high yield and purity (2007), Protein Expr. Purif., 52, 373-378.
    View publication on PubMed
Search for more literature for 5.3.1.9

Cloned(Commentary)

Cloned (Comment) Organism
-
 Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
additional information recombinant enzyme carrying His-tag shows enzymatic acitivity equal to native enzyme Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
6-phosphogluconate pH 7.6, 25°C Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
 D-fructose 6-phosphate pH 7.6, 25°C Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme with His-tag. Rapid purification by Ni-NTA affinity chromatography with 80% yield Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 6-phosphate
-
 Mycobacterium tuberculosis D-glucose 6-phosphate
-
 ?
D-fructose 6-phosphate
-
 Mycobacterium tuberculosis H37Rv D-glucose 6-phosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 stable up to Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6 9
-
 Mycobacterium tuberculosis