Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Thermococcus litoralis |
Protein Variants | Comment | Organism |
---|---|---|
E98V | the ratio of turnover number and Km-value is 31373fold lower than that of the wild-type enzyme. The absorption maximum at 420 nm as found in wild-type enzyme is completely lost. Mutant enzyme does not contain iron or zinc or other metal | Thermococcus litoralis |
H137A | the ratio of turnover number and Km-value is 66.7fold lower than that of the wild-type enzyme. The absorption maximum at 420 nm as found in wild-type enzyme is completely lost. Mutant enzyme does not contain iron or zinc or other metal | Thermococcus litoralis |
H89A | the ratio of turnover number and Km-value is 2712fold lower than that of the wild-type enzyme. The absorption maximum at 420 nm as found in wild-type enzyme is completely lost. Mutant enzyme does not contain iron or zinc or other metal | Thermococcus litoralis |
H91A | the ratio of turnover number and Km-value is 66.7fold lower than that of the wild-type enzyme. The absorption maximum at 420 nm as found in wild-type enzyme is completely lost. Mutant enzyme does not contain iron or zinc or other metal | Thermococcus litoralis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 10 mM, 96% inhibition, D-fructose 6-phosphate as substrate | Thermococcus litoralis | |
Co2+ | 10 mM, 59% inhibition, D-fructose 6-phosphate as substrate | Thermococcus litoralis | |
Cu2+ | 10 mM, 96% inhibition, D-fructose 6-phosphate as substrate | Thermococcus litoralis | |
D-fructose 1,6-bisphosphate | - |
Thermococcus litoralis | |
D-Fructose 1-phosphate | - |
Thermococcus litoralis | |
D-gluconate 6-phosphate | - |
Thermococcus litoralis | |
D-mannose 6-phosphate | - |
Thermococcus litoralis | |
EDTA | 10 mM, 78% inhibition, D-fructose 6-phosphate as substrate | Thermococcus litoralis | |
K+ | 10 mM, 18% inhibition, D-fructose 6-phosphate as substrate | Thermococcus litoralis | |
Mn2+ | 10 mM, 18% inhibition, D-fructose 6-phosphate as substrate | Thermococcus litoralis | |
Ni2+ | 10 mM, 84% inhibition, D-fructose 6-phosphate as substrate | Thermococcus litoralis | |
Zn2+ | 10 mM, 89% inhibition, D-fructose 6-phosphate as substrate | Thermococcus litoralis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.59 | - |
D-fructose 6-phosphate | 50°C, pH 7.5, mutant enzyme E89V | Thermococcus litoralis | |
1.4 | - |
D-fructose 6-phosphate | 50°C, pH 7.5, mutant enzyme H137A | Thermococcus litoralis | |
1.7 | - |
D-fructose 6-phosphate | 50°C, pH 7.5, wild-type enzyme | Thermococcus litoralis | |
2.1 | - |
D-fructose 6-phosphate | 50°C, pH 7.5, mutant enzyme H91A | Thermococcus litoralis | |
3.5 | - |
D-fructose 6-phosphate | 50°C, pH 7.5, mutant enzyme H89A | Thermococcus litoralis | |
11.7 | - |
D-glucose 6-phosphate | 50°C, pH 7.5, wild-type enzyme | Thermococcus litoralis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 10 mM, 37°C, 156% enhancement of the activity of the wild-type enzyme, mutant enzymes H89A, H91A, E98V and H137A do not contain iron or zinc | Thermococcus litoralis | |
Iron | wild-type enzyme contains 1.25 mol iron per mol of dimer, mutant enzymes H89A, H91A, E98V and H137A do not contain iron or zinc | Thermococcus litoralis | |
Zinc | wild-type enzyme contains 0.24 mol zinc per mol of dimer | Thermococcus litoralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus litoralis | P84140 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus litoralis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
47.3 | - |
formation of D-glucose 6-phosphate | Thermococcus litoralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fructose 6-phosphate | - |
Thermococcus litoralis | D-glucose 6-phosphate | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
D-fructose 6-phosphate | 50°C, pH 7.5, mutant enzyme E98V | Thermococcus litoralis | |
0.021 | - |
D-fructose 6-phosphate | 50°C, pH 7.5, mutant enzyme H89A | Thermococcus litoralis | |
0.34 | - |
D-fructose 6-phosphate | 50°C, pH 7.5, mutant enzyme H137A | Thermococcus litoralis | |
0.5 | - |
D-fructose 6-phosphate | 50°C, pH 7.5, mutant enzyme H91A | Thermococcus litoralis | |
26 | - |
D-fructose 6-phosphate | 50°C, pH 7.5, wild-type enzyme | Thermococcus litoralis | |
42 | - |
D-glucose 6-phosphate | 50°C, pH 7.5, wild-type enzyme | Thermococcus litoralis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
D-gluconate 6-phosphate | 50°C, pH 7.5, D-fructose 6-phosphate as substrate | Thermococcus litoralis | |
2.3 | - |
D-mannose 6-phosphate | 50°C, pH 7.5, D-fructose 6-phosphate as substrate | Thermococcus litoralis | |
4 | - |
D-Fructose 1-phosphate | 50°C, pH 7.5, D-fructose 6-phosphate as substrate | Thermococcus litoralis | |
12 | - |
D-fructose 1,6-bisphosphate | 50°C, pH 7.5, D-fructose 6-phosphate as substrate | Thermococcus litoralis |