Literature summary for 5.3.1.8 extracted from

Hu, L.; Li, H.; Qin, R.; Xu, R.; Li, J.; Li, L.; Wei, P.; Yang, J.
Plant phosphomannose isomerase as a selectable marker for rice transformation (2016), Sci. Rep., 6, 25921 .

Search for more literature for 5.3.1.8

Application

Application	Comment	Organism
synthesis	use as a selectable marker gene for transformation of Oryza sativa. In both Indica and Japonica subspecies, PMI expression can select and produce transgenic plants in a pattern similar to that of Escherichia coli PMI. The transgenic plants exhibit an accumulation of plant PMI transcripts and enhancement of the in vivo PMI activity. A gene of interest is successfully transformed into rice using the plant PMI as selectable marker gene	Oryza sativa Japonica Group
synthesis	use as a selectable marker gene for transformation of Oryza sativa. In both Indica and Japonica subspecies, PMI expression can select and produce transgenic plants in a pattern similar to that of Escherichia coli PMI. The transgenic plants exhibit an accumulation of plant PMI transcripts and enhancement of the in vivo PMI activity. A gene of interest is successfully transformed into rice using the plant PMI as selectable marker gene	Chlorella variabilis

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Oryza sativa Japonica Group
expression in Escherichia coli	Chlorella variabilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.625	-	D-mannose 6-phosphate	pH 7.5, 30°C	Chlorella variabilis
3.808	-	D-mannose 6-phosphate	pH 7.5, 30°C	Oryza sativa Japonica Group
6.462	-	D-mannose 6-phosphate	pH 7.5, 30°C	Oryza sativa Japonica Group

Organism

Organism	UniProt	Comment	Textmining
Chlorella variabilis	E1ZPT8	-	-
Oryza sativa Japonica Group	Q6H5C7	-	-
Oryza sativa Japonica Group	Q9FTR6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Mannose 6-phosphate	-	Oryza sativa Japonica Group	D-Fructose 6-phosphate	-	?
D-Mannose 6-phosphate	-	Chlorella variabilis	D-Fructose 6-phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 44180, calculated from sequence	Oryza sativa Japonica Group
?	x * 45850, calculated from sequence	Chlorella variabilis
?	x * 46930, calculated from sequence	Oryza sativa Japonica Group

Synonyms

Synonyms	Comment	Organism
CHLNCDRAFT_139231	-	Chlorella variabilis
Os01g0127900	-	Oryza sativa Japonica Group
Os09g0389000	-	Oryza sativa Japonica Group

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Release 2023.1 (January 2023)