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Literature summary for 5.3.1.8 extracted from

  • Akutsu, J.; Zhang, Z.; Morita, R.; Kawarabayasi, Y.
    Identification and characterization of a thermostable bifunctional enzyme with phosphomannose isomerase and sugar-1-phosphate nucleotidylyltransferase activities from a hyperthermophilic archaeon, Pyrococcus horikoshii OT3 (2015), Extremophiles, 19, 1077-1085.
    View publication on PubMed
Search for more literature for 5.3.1.8

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pyrococcus horikoshii

Inhibitors

Inhibitors Comment Organism Structure
EDTA 0.1 mM, about 50% inhibition Pyrococcus horikoshii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0986
-
 beta-D-mannose 6-phosphate pH 7.6, 65°C Pyrococcus horikoshii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 2 mM, maximum activity Pyrococcus horikoshii
Co2+ 2 mM, about 80% of the activity with Cu2+ Pyrococcus horikoshii
Mn2+ 2 mM, about 55% of the activity with Cu2+ Pyrococcus horikoshii
additional information absolute requirement for divalent cation Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52900
-
 x * 52900, SDS-PAGE Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O58649 bifunctional enzyme with mannose-6-phosphate isomerase and sugar-1-phosphate nucleotidylyltransferase activities, EC 5.3.1.8 and EC 2.7.7.13, respectively
-
Pyrococcus horikoshii DSM 12428 O58649 bifunctional enzyme with mannose-6-phosphate isomerase and sugar-1-phosphate nucleotidylyltransferase activities, EC 5.3.1.8 and EC 2.7.7.13, respectively
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-mannose 6-phosphate
-
 Pyrococcus horikoshii beta-D-fructose 6-phosphate
-
 ?
beta-D-mannose 6-phosphate
-
 Pyrococcus horikoshii DSM 12428 beta-D-fructose 6-phosphate
-
 ?
beta-D-mannose 6-phosphate
-
 Pyrococcus horikoshii ATCC 700860 beta-D-fructose 6-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 52900, SDS-PAGE Pyrococcus horikoshii
More mannose-6-phosphate isomerase activity is abolished by deletion of the C-terminal 14 residues. The N-terminal region plays an important role in the solubility of the entire protein and in the acquisition of the correct structure Pyrococcus horikoshii

Synonyms

Synonyms Comment Organism
PH0925
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
 Pyrococcus horikoshii