Application | Comment | Organism |
---|---|---|
synthesis | mannose-6-phosphate isomerase from Bacillus subtilis is applied for the production of L-ribose by direct isomerization of L-ribulose | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain ER2566 | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | complete inactivation | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
110 | - |
D-ribose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
312 | - |
L-allose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
433 | - |
D-Lyxose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
469 | - |
D-talose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
946 | - |
D-mannose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
998 | - |
L-ribose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | activates | Bacillus subtilis | |
Ca2+ | activates | Bacillus subtilis | |
Co2+ | activates, best metal ion, maximal activity at 0.5 mM | Bacillus subtilis | |
Cu2+ | activates | Bacillus subtilis | |
Mg2+ | activates | Bacillus subtilis | |
Mn2+ | highly activating | Bacillus subtilis | |
additional information | mannose-6-phosphate isomerases are metalloenzymes that require a divalent ion metal cofactor for activity and catalysis | Bacillus subtilis | |
Ni2+ | activates | Bacillus subtilis | |
Zn2+ | activates | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36444 | - |
* 36500, recombinant enzyme, SDS-PAGE, 1 * 36444, sequence calculation | Bacillus subtilis |
36500 | - |
* 36500, recombinant enzyme, SDS-PAGE, 1 * 36444, sequence calculation | Bacillus subtilis |
36600 | - |
gel filtration, recombinant enzyme | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Mannose 6-phosphate | Bacillus subtilis | - |
D-Fructose 6-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | Q9AGZ4 | enzyme from Bacillus subtilis strain HB002; ATCC 23857 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 27fold from Escherichia coli strain ER2566 by affinity chromatography | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificity, overview | Bacillus subtilis |
0.0101 | - |
recombinant enzyme | Bacillus subtilis |
0.0165 | - |
recombinant enzyme | Bacillus subtilis |
0.0225 | - |
recombinant enzyme | Bacillus subtilis |
0.0659 | - |
recombinant enzyme | Bacillus subtilis |
0.0918 | - |
recombinant enzyme | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-allose | - |
Bacillus subtilis | D-psicose | - |
r | |
D-Lyxose | - |
Bacillus subtilis | D-Xylulose | - |
r | |
D-Mannose | - |
Bacillus subtilis | D-Fructose | - |
r | |
D-Mannose 6-phosphate | - |
Bacillus subtilis | D-Fructose 6-phosphate | - |
r | |
D-ribose | - |
Bacillus subtilis | D-ribulose | - |
r | |
D-talose | - |
Bacillus subtilis | D-tagatose | - |
r | |
L-allose | - |
Bacillus subtilis | L-psicose | - |
r | |
L-lyxose | - |
Bacillus subtilis | L-xylulose | - |
r | |
L-Mannose | - |
Bacillus subtilis | L-Fructose | - |
r | |
L-ribose | - |
Bacillus subtilis | L-ribulose | - |
r | |
L-ribulose | best substrate | Bacillus subtilis | L-ribose | - |
r | |
L-talose | - |
Bacillus subtilis | L-tagatose | - |
r | |
additional information | the enzyme is specific for aldose substrates possessing hydroxyl groups oriented in the same direction at the C-2 and C-3 positions, such as the D- and L-enantiomers of ribose, lyxose, talose, mannose, and allose, substrate specificity, overview | Bacillus subtilis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | * 36500, recombinant enzyme, SDS-PAGE, 1 * 36444, sequence calculation | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
recombinant enzyme | Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | - |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
first-order kinetics for thermal inactivation | Bacillus subtilis |
25 | - |
purified recombinant enzyme, half-life is 461 h | Bacillus subtilis |
30 | - |
purified recombinant enzyme, half-life is 325 h | Bacillus subtilis |
35 | - |
purified recombinant enzyme, half-life is 236 h | Bacillus subtilis |
40 | - |
purified recombinant enzyme, half-life is 111 h | Bacillus subtilis |
45 | - |
purified recombinant enzyme, half-life is 56 h | Bacillus subtilis |
50 | - |
purified recombinant enzyme, half-life is 10 h | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
72 | - |
D-ribose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
920 | - |
L-allose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
3748 | - |
D-mannose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
7089 | - |
D-Lyxose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
17600 | - |
L-ribose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
63970 | - |
D-talose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
recombinant enzyme | Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | - |
Bacillus subtilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
D-ribose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
2.9 | - |
L-allose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
3.5 | - |
D-mannose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
6.8 | - |
D-talose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
16.4 | - |
D-Lyxose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
17.6 | - |
L-ribose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis |