Literature summary for 5.3.1.8 extracted from

Maruta, T.; Yonemitsu, M.; Yabuta, Y.; Tamoi, M.; Ishikawa, T.; Shigeoka, S.
Arabidopsis phosphomannose isomerase 1, but not phosphomannose isomerase 2, is essential for ascorbic acid biosynthesis (2008), J. Biol. Chem., 283, 28842-28851.

Search for more literature for 5.3.1.8

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
additional information	knockout of isoform Pmi2 does not affect the total ascorbic acid levels in leaves	Arabidopsis thaliana
additional information	reduction of Pmi1 expression by RNA interference results in substantial decrease in the total ascorbic acid content of leaves	Arabidopsis thaliana

Inhibitors

Inhibitors	Comment	Organism	Structure
Cd2+	1 mM, no residual activity	Arabidopsis thaliana
dithiothreitol	1 mM, 73% residual activity; 1 mM, 84% residual activity	Arabidopsis thaliana
EDTA	2.5 mM, 54% residual activity; 2.5 mM, 86% residual activity	Arabidopsis thaliana
L-ascorbic acid	1 mM, 77% residual activity	Arabidopsis thaliana
p-chloromercuribenzoate	0.05 mM, 33% residual activity; 0.05 mM, 39% residual activity	Arabidopsis thaliana
Zn2+	1 mM, no residual activity	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0413	-	D-mannose 6-phosphate	pH 7.5, 25°C	Arabidopsis thaliana
0.372	-	D-mannose 6-phosphate	pH 7.5, 25°C	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	isoform Pmi1	-
Arabidopsis thaliana	-	isoform Pmi2	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	induction of expression under continuous light. Diurnal expression pattern in parallel with the total Pmi1 activity and the total ascorbic acid content in leaf	Arabidopsis thaliana	-
additional information	constitutive expression in both vegetative and reproductive organs	Arabidopsis thaliana	-
additional information	no expression in any organ under light. Under long term darkness, expression of isoform Pmi2 with concomitant decrease in ascorbic acid levels	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Mannose 6-phosphate	-	Arabidopsis thaliana	D-Fructose 6-phosphate	-	r

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
52	-	-	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Arabidopsis thaliana

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)