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Literature summary for 5.3.1.8 extracted from

  • Lee, H.J.; Chang, H.Y.; Venkatesan, N.; Peng, H.L.
    Identification of amino acid residues important for the phosphomannose isomerase activity of PslB in Pseudomonas aeruginosa PAO1 (2008), FEBS Lett., 582, 3479-3483.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
diphosphate 0.5 mM, 120% of initial activity Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
E410A about 25% of wild-type catalytic efficiency Pseudomonas aeruginosa
E458A about 25% of wild-type catalytic efficiency Pseudomonas aeruginosa
H411A about 25% of wild-type catalytic efficiency Pseudomonas aeruginosa
N433A about 25% of wild-type catalytic efficiency Pseudomonas aeruginosa
R373A about 4% of wild-type catalytic efficiency Pseudomonas aeruginosa
R408A complete loss of phosphomannose isomerase activity without affecting guanosine diphosphate-D-mannose diphosphorylase activity Pseudomonas aeruginosa
R408K complete loss of phosphomannose isomerase activity without affecting guanosine diphosphate-D-mannose diphosphorylase activity Pseudomonas aeruginosa
R472A about 1% of wild-type catalytic efficiency Pseudomonas aeruginosa
R479A about 3% of wild-type catalytic efficiency Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
2,3-Butanedione inactivation Pseudomonas aeruginosa
ADP-glucose 0.5 mM, 77.8% of initial activity Pseudomonas aeruginosa
D-mannose 1-phosphate 0.5 mM, 81.9% of initial activity Pseudomonas aeruginosa
GDP-mannose 0.5 mM, 46.7% of initial activity Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18
-
D-mannose 6-phosphate wild-type, pH 7.0, 25°C Pseudomonas aeruginosa
9.04
-
D-mannose 6-phosphate mutant E410A, pH 7.0, 25°C Pseudomonas aeruginosa
12.28
-
D-mannose 6-phosphate mutant R479A, pH 7.0, 25°C Pseudomonas aeruginosa
12.36
-
D-mannose 6-phosphate mutant E458A, pH 7.0, 25°C Pseudomonas aeruginosa
12.9
-
D-mannose 6-phosphate mutant N433A, pH 7.0, 25°C Pseudomonas aeruginosa
15.9
-
D-mannose 6-phosphate mutant R373A, pH 7.0, 25°C Pseudomonas aeruginosa
16.26
-
D-mannose 6-phosphate mutant H411A, pH 7.0, 25°C Pseudomonas aeruginosa
23.12
-
D-mannose 6-phosphate mutant R472A, pH 7.0, 25°C Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ required for phosphomannose isomerase activity Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
bifunctional enzyme with phosphomannose isomerase and guanosine diphosphate-D-mannose diphosphorylase activities
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Mannose 6-phosphate
-
Pseudomonas aeruginosa D-Fructose 6-phosphate
-
r

Synonyms

Synonyms Comment Organism
PslB
-
Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.25
-
D-mannose 6-phosphate mutant R472A, pH 7.0, 25°C Pseudomonas aeruginosa
0.54
-
D-mannose 6-phosphate mutant R479A, pH 7.0, 25°C Pseudomonas aeruginosa
0.75
-
D-mannose 6-phosphate mutant R373A, pH 7.0, 25°C Pseudomonas aeruginosa
1.38
-
D-mannose 6-phosphate wild-type, pH 7.0, 25°C Pseudomonas aeruginosa
2.63
-
D-mannose 6-phosphate mutant E410A, pH 7.0, 25°C Pseudomonas aeruginosa
3.25
-
D-mannose 6-phosphate mutant E458A, pH 7.0, 25°C Pseudomonas aeruginosa
3.5
-
D-mannose 6-phosphate mutant N433A, pH 7.0, 25°C Pseudomonas aeruginosa
4.41
-
D-mannose 6-phosphate mutant H411A, pH 7.0, 25°C Pseudomonas aeruginosa