Activating Compound | Comment | Organism | Structure |
---|---|---|---|
diphosphate | 0.5 mM, 120% of initial activity | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
E410A | about 25% of wild-type catalytic efficiency | Pseudomonas aeruginosa |
E458A | about 25% of wild-type catalytic efficiency | Pseudomonas aeruginosa |
H411A | about 25% of wild-type catalytic efficiency | Pseudomonas aeruginosa |
N433A | about 25% of wild-type catalytic efficiency | Pseudomonas aeruginosa |
R373A | about 4% of wild-type catalytic efficiency | Pseudomonas aeruginosa |
R408A | complete loss of phosphomannose isomerase activity without affecting guanosine diphosphate-D-mannose diphosphorylase activity | Pseudomonas aeruginosa |
R408K | complete loss of phosphomannose isomerase activity without affecting guanosine diphosphate-D-mannose diphosphorylase activity | Pseudomonas aeruginosa |
R472A | about 1% of wild-type catalytic efficiency | Pseudomonas aeruginosa |
R479A | about 3% of wild-type catalytic efficiency | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-Butanedione | inactivation | Pseudomonas aeruginosa | |
ADP-glucose | 0.5 mM, 77.8% of initial activity | Pseudomonas aeruginosa | |
D-mannose 1-phosphate | 0.5 mM, 81.9% of initial activity | Pseudomonas aeruginosa | |
GDP-mannose | 0.5 mM, 46.7% of initial activity | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.18 | - |
D-mannose 6-phosphate | wild-type, pH 7.0, 25°C | Pseudomonas aeruginosa | |
9.04 | - |
D-mannose 6-phosphate | mutant E410A, pH 7.0, 25°C | Pseudomonas aeruginosa | |
12.28 | - |
D-mannose 6-phosphate | mutant R479A, pH 7.0, 25°C | Pseudomonas aeruginosa | |
12.36 | - |
D-mannose 6-phosphate | mutant E458A, pH 7.0, 25°C | Pseudomonas aeruginosa | |
12.9 | - |
D-mannose 6-phosphate | mutant N433A, pH 7.0, 25°C | Pseudomonas aeruginosa | |
15.9 | - |
D-mannose 6-phosphate | mutant R373A, pH 7.0, 25°C | Pseudomonas aeruginosa | |
16.26 | - |
D-mannose 6-phosphate | mutant H411A, pH 7.0, 25°C | Pseudomonas aeruginosa | |
23.12 | - |
D-mannose 6-phosphate | mutant R472A, pH 7.0, 25°C | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | required for phosphomannose isomerase activity | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
bifunctional enzyme with phosphomannose isomerase and guanosine diphosphate-D-mannose diphosphorylase activities | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Mannose 6-phosphate | - |
Pseudomonas aeruginosa | D-Fructose 6-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PslB | - |
Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
D-mannose 6-phosphate | mutant R472A, pH 7.0, 25°C | Pseudomonas aeruginosa | |
0.54 | - |
D-mannose 6-phosphate | mutant R479A, pH 7.0, 25°C | Pseudomonas aeruginosa | |
0.75 | - |
D-mannose 6-phosphate | mutant R373A, pH 7.0, 25°C | Pseudomonas aeruginosa | |
1.38 | - |
D-mannose 6-phosphate | wild-type, pH 7.0, 25°C | Pseudomonas aeruginosa | |
2.63 | - |
D-mannose 6-phosphate | mutant E410A, pH 7.0, 25°C | Pseudomonas aeruginosa | |
3.25 | - |
D-mannose 6-phosphate | mutant E458A, pH 7.0, 25°C | Pseudomonas aeruginosa | |
3.5 | - |
D-mannose 6-phosphate | mutant N433A, pH 7.0, 25°C | Pseudomonas aeruginosa | |
4.41 | - |
D-mannose 6-phosphate | mutant H411A, pH 7.0, 25°C | Pseudomonas aeruginosa |