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Literature summary for 5.3.1.8 extracted from

  • Degenhardt, J.; Poppe, A.; Montag, J.; Szankowski, I.
    The use of the phosphomannose-isomerase/mannose selection system to recover transgenic apple plants (2006), Plant Cell Rep., 25, 1149-1156.
    View publication on PubMed

Application

Application Comment Organism
agriculture expression system based on phosphomannose-isomerase gene as a selectable marker in Agrobacterium-based transformation and mannose as the selective agent for the transformation of apple. Selection of leaf explants on medium supplemented with mannose and sorbitol, integration of transgenes in the apple genome and their activity are confirmed Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
use of enzyme as selectable marker Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
expression in Malus domestica
-