BRENDA - Enzyme Database
show all sequences of 5.3.1.6

Substrate specificity of a recombinant ribose-5-phosphate isomerase from Streptococcus pneumoniae and its application in the production of l-lyxose and l-tagatose

Park, C.; Yeom, S.; Lim, Y.; Kim, Y.; Oh, D.; World J. Microbiol. Biotechnol. 27, 743-750 (2011)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
RpiB is a potential producer of L-form monosaccharides
Streptococcus pneumoniae
Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli ER2566 cells
Streptococcus pneumoniae
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
the enzyme is not activated by monovalent or divalent cations
Streptococcus pneumoniae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23724
-
4 * 23724, calculated from amino acid sequence
Streptococcus pneumoniae
24000
-
4 * 24000, SDS-PAGE
Streptococcus pneumoniae
96000
-
gel filtration
Streptococcus pneumoniae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
D-ribose 5-phosphate
Streptococcus pneumoniae
highest specific activity
D-ribulose 5-phosphate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Streptococcus pneumoniae
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
HiTrap Q anion exchange column chromatography and Sephacryl S-300 gel filtration
Streptococcus pneumoniae
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0005
-
using D-sorbose as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.0011
-
using L-fructose as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.0013
-
using L-tagatose as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.0019
-
using D-allose as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.0021
-
using D-psicose as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.0025
-
using D-ribose as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.0053
-
using D-gulose as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.0096
-
using L-lyxose as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.019
-
using L-talose as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.027
-
using L-lyxose as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.048
-
using D-ribulose as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.058
-
using D-glucose 6-phosphate as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.121
-
using L-xylulose as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.388
-
using D-ribose 5-phosphate as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
D-Allose
-
717008
Streptococcus pneumoniae
D-Psicose
-
-
-
r
D-Glucose 6-phosphate
-
717008
Streptococcus pneumoniae
?
-
-
-
?
D-gulose
-
717008
Streptococcus pneumoniae
D-sorbose
-
-
-
r
D-psicose
-
717008
Streptococcus pneumoniae
D-allose
-
-
-
r
D-Ribose
-
717008
Streptococcus pneumoniae
D-Ribulose
-
-
-
r
D-ribose 5-phosphate
highest specific activity
717008
Streptococcus pneumoniae
D-ribulose 5-phosphate
-
-
-
?
D-ribulose
-
717008
Streptococcus pneumoniae
D-ribose
-
-
-
r
D-sorbose
lowest activity with L-sorbose
717008
Streptococcus pneumoniae
D-gulose
-
-
-
r
L-fructose
-
717008
Streptococcus pneumoniae
L-mannose
-
-
-
r
L-Lyxose
particularly high activity with L-lyxose
717008
Streptococcus pneumoniae
L-Xylulose
-
-
-
r
L-Mannose
-
717008
Streptococcus pneumoniae
L-Fructose
-
-
-
r
L-tagatose
particularly high activity with L-tagatose
717008
Streptococcus pneumoniae
L-talose
-
-
-
r
L-talose
particularly high activity with L-talose
717008
Streptococcus pneumoniae
L-tagatose
-
-
-
r
L-xylulose
particularly high activity with L-lyxose
717008
Streptococcus pneumoniae
L-lyxose
-
-
-
r
additional information
no activity is observed with D-mannose-6-phosphate
717008
Streptococcus pneumoniae
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 24000, SDS-PAGE
Streptococcus pneumoniae
homotetramer
4 * 23724, calculated from amino acid sequence
Streptococcus pneumoniae
Synonyms
Synonyms
Commentary
Organism
ribose-5-phosphate isomerase
-
Streptococcus pneumoniae
RpiB
-
Streptococcus pneumoniae
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Streptococcus pneumoniae
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
45
-
Streptococcus pneumoniae
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
50
enzyme activity is stable for 48 h at 35°C, and shows half-lives of 15 h at 40°C, and 6 h at 50°C
Streptococcus pneumoniae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Streptococcus pneumoniae
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
8.5
-
Streptococcus pneumoniae
Application (protein specific)
Application
Commentary
Organism
synthesis
RpiB is a potential producer of L-form monosaccharides
Streptococcus pneumoniae
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli ER2566 cells
Streptococcus pneumoniae
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
the enzyme is not activated by monovalent or divalent cations
Streptococcus pneumoniae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23724
-
4 * 23724, calculated from amino acid sequence
Streptococcus pneumoniae
24000
-
4 * 24000, SDS-PAGE
Streptococcus pneumoniae
96000
-
gel filtration
Streptococcus pneumoniae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
D-ribose 5-phosphate
Streptococcus pneumoniae
highest specific activity
D-ribulose 5-phosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
HiTrap Q anion exchange column chromatography and Sephacryl S-300 gel filtration
Streptococcus pneumoniae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0005
-
using D-sorbose as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.0011
-
using L-fructose as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.0013
-
using L-tagatose as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.0019
-
using D-allose as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.0021
-
using D-psicose as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.0025
-
using D-ribose as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.0053
-
using D-gulose as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.0096
-
using L-lyxose as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.019
-
using L-talose as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.027
-
using L-lyxose as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.048
-
using D-ribulose as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.058
-
using D-glucose 6-phosphate as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.121
-
using L-xylulose as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
0.388
-
using D-ribose 5-phosphate as substrate, at 35°C and pH 7.5
Streptococcus pneumoniae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
D-Allose
-
717008
Streptococcus pneumoniae
D-Psicose
-
-
-
r
D-Glucose 6-phosphate
-
717008
Streptococcus pneumoniae
?
-
-
-
?
D-gulose
-
717008
Streptococcus pneumoniae
D-sorbose
-
-
-
r
D-psicose
-
717008
Streptococcus pneumoniae
D-allose
-
-
-
r
D-Ribose
-
717008
Streptococcus pneumoniae
D-Ribulose
-
-
-
r
D-ribose 5-phosphate
highest specific activity
717008
Streptococcus pneumoniae
D-ribulose 5-phosphate
-
-
-
?
D-ribulose
-
717008
Streptococcus pneumoniae
D-ribose
-
-
-
r
D-sorbose
lowest activity with L-sorbose
717008
Streptococcus pneumoniae
D-gulose
-
-
-
r
L-fructose
-
717008
Streptococcus pneumoniae
L-mannose
-
-
-
r
L-Lyxose
particularly high activity with L-lyxose
717008
Streptococcus pneumoniae
L-Xylulose
-
-
-
r
L-Mannose
-
717008
Streptococcus pneumoniae
L-Fructose
-
-
-
r
L-tagatose
particularly high activity with L-tagatose
717008
Streptococcus pneumoniae
L-talose
-
-
-
r
L-talose
particularly high activity with L-talose
717008
Streptococcus pneumoniae
L-tagatose
-
-
-
r
L-xylulose
particularly high activity with L-lyxose
717008
Streptococcus pneumoniae
L-lyxose
-
-
-
r
additional information
no activity is observed with D-mannose-6-phosphate
717008
Streptococcus pneumoniae
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 24000, SDS-PAGE
Streptococcus pneumoniae
homotetramer
4 * 23724, calculated from amino acid sequence
Streptococcus pneumoniae
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Streptococcus pneumoniae
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
45
-
Streptococcus pneumoniae
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
50
enzyme activity is stable for 48 h at 35°C, and shows half-lives of 15 h at 40°C, and 6 h at 50°C
Streptococcus pneumoniae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Streptococcus pneumoniae
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
8.5
-
Streptococcus pneumoniae
Other publictions for EC 5.3.1.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748388
Lee
Biotransformation of fructose ...
Hungateiclostridium thermocellum, Hungateiclostridium thermocellum ATCC 27405
J. Microbiol. Biotechnol.
28
418-424
2018
-
1
1
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
1
1
-
2
-
1
-
-
-
-
-
-
-
1
1
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-
-
-
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-
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-
-
-
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
749044
Chou
Identification of a noncanoni ...
Homo sapiens
PLoS Biol.
16
e2003714
2018
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
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3
-
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1
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1
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3
-
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-
-
-
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-
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-
-
-
-
748429
de V. C. Sinatti
In silico identification of i ...
Trypanosoma cruzi, Trypanosoma cruzi CL Brener
J. Mol. Graph. Model.
77
168-180
2017
-
-
-
-
-
-
2
-
-
-
-
-
-
8
-
-
-
-
-
-
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1
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2
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-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
747491
Heintze
Ribose 5-phosphate isomerase ...
Homo sapiens
Cell. Signal.
28
1380-1388
2016
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
749079
Kaur
Mutational and structural ana ...
Leishmania donovani
PLoS ONE
11
e0150764
2016
-
-
1
1
8
-
-
5
-
-
-
-
-
6
-
-
-
-
-
1
-
-
6
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
-
1
8
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
5
-
-
-
-
-
-
-
-
5
5
749320
Faria
Disclosing the essentiality o ...
Leishmania infantum
Sci. Rep.
6
26937
2016
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
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-
-
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-
-
-
-
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-
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-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
748426
Capriles
Structural modeling and docki ...
Homo sapiens, Leishmania major
J. Mol. Graph. Model.
55
134-147
2015
-
-
-
2
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
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3
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2
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-
-
-
-
-
-
-
-
-
-
727231
Feng
Characterization of ribose-5-p ...
Pseudothermotoga lettingae, Pseudothermotoga lettingae TMO
Biotechnol. Lett.
35
719-724
2013
-
-
1
-
-
-
-
1
-
1
3
2
-
7
-
-
1
-
-
-
1
-
4
2
1
1
-
1
1
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
3
2
-
-
-
1
-
-
1
-
4
2
1
-
1
1
1
1
-
-
-
-
-
-
1
1
728196
Wang
Concerted proton transfer mech ...
Hungateiclostridium thermocellum
J. Phys. Chem. B
117
9354-9361
2013
-
-
-
-
-
-
-
-
-
-
-
1
-
9
-
-
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1
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1
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1
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1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
726618
Wang
Preliminary crystallographic a ...
Streptococcus mutans
Acta Crystallogr. Sect. F
68
565-568
2012
-
-
2
2
-
-
-
-
-
-
2
2
-
6
-
-
2
-
-
-
-
-
2
2
4
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
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-
-
-
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2
2
-
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-
2
-
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-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
728735
Sun
Thermophilic Thermotoga mariti ...
Thermotoga maritima
Protein Expr. Purif.
82
302-307
2012
-
-
1
-
-
1
-
2
-
-
2
1
-
6
-
-
1
-
-
-
2
-
1
2
2
1
1
1
2
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
2
-
-
2
1
-
-
-
1
-
-
2
-
1
2
1
1
1
2
1
-
-
-
-
-
-
-
-
-
713894
Yeom
Increased D-allose production ...
Hungateiclostridium thermocellum, Hungateiclostridium thermocellum ATCC 27405
Appl. Microbiol. Biotechnol.
89
1859-1866
2011
-
-
1
-
17
-
-
7
-
-
-
-
-
8
-
-
1
-
-
-
9
-
4
-
2
1
1
1
7
1
1
-
-
-
-
-
-
-
1
-
-
17
-
-
-
-
7
-
-
-
-
-
-
-
1
-
-
9
-
4
-
1
1
1
7
1
1
-
-
-
-
-
-
7
7
713908
Jung
Crystal structure of Clostridi ...
Hungateiclostridium thermocellum, Hungateiclostridium thermocellum ATCC 27405
Appl. Microbiol. Biotechnol.
90
517-527
2011
-
-
1
1
-
-
-
-
-
-
1
-
-
9
-
-
1
-
-
-
-
-
8
1
2
-
-
-
-
-
-
-
-
-
-
-
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Response of some Calvin cycle ...
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1997
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2794
Martin
Microsequencing and cDNA cloni ...
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Plant Mol. Biol.
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2793
Malaisse
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Diastereotopic specificity of ...
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Med. Sci. Res.
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2789
Skrukrud
Chloroplast phosphoribulokinas ...
Cyberlindnera jadinii
FEBS Lett.
280
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1991
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2792
Skrukrud
Purification and characterizat ...
Pisum sativum
Plant Physiol.
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1991
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2791
Cronin
The enzymes of the classical p ...
Trypanosoma brucei
FEBS Lett.
244
26-30
1989
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2797
Horitsu
-
Die Isolierung und der Bestand ...
Sus scrofa
Bull. Tokyo Kasei University
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1988
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2790
Anderson
Ribose-5-phosphate isomerase a ...
Cyberlindnera jadinii
FEBS Lett.
212
45-48
1987
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2788
Sainis
The association of ribulose-1, ...
Pisum sativum
Biochem. Biophys. Res. Commun.
139
947-954
1986
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2778
Domagk
-
Purification and properties of ...
Bos taurus
Hoppe-Seyler's Z. Physiol. Chem.
355
781-786
1984
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2787
MacElroy
Bacterial ribosephosphate isom ...
[Bacillus] caldolyticus, Chromatium sp., Escherichia coli, Rhodospirillum rubrum, Halothiobacillus neapolitanus, Thiobacillus thioparus, Chromatium sp. D
Methods Enzymol.
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1982
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2785
Horitsu
-
Chromatographic purification o ...
Cyberlindnera jadinii
Agric. Biol. Chem.
43
663-664
1979
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2786
Woodruff
Inhibition of ribose-5-phospha ...
Spinacia oleracea
J. Biol. Chem.
254
5866-5867
1979
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2784
Horitsu
-
Three forms of Candida utilis ...
Cyberlindnera jadinii
Agric. Biol. Chem.
42
2195-2201
1978
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5
3
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2782
Horitsu
-
Purification, properties and s ...
Cyberlindnera jadinii
Agric. Biol. Chem.
40
257-264
1976
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10
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1
1
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1
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2783
Middaugh
The effect of temperature on r ...
[Bacillus] caldolyticus, Thiobacillus thioparus
J. Biochem.
79
1331-1344
1976
4
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4
16
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12
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16
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2
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4
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2
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2
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2779
Essenberg
Two ribose-5-phosphate isomera ...
Escherichia coli
Eur. J. Biochem.
55
323-332
1975
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3
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-
-
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2780
Domagk
D-Ribose-5-phosphate isomerase ...
Bos taurus
Methods Enzymol.
41A
424-426
1975
-
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2
1
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1
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1
1
1
1
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-
-
-
-
2781
Domagk
D-Ribose-5-phosphate isomerase ...
Cyberlindnera jadinii
Methods Enzymol.
41B
427-429
1975
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1
2
1
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1
1
1
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1
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-
-
-
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-
2777
Wood
The detection and occurence of ...
Rattus norvegicus, Spinacia oleracea
Arch. Biochem. Biophys.
160
40-46
1974
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1
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10
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10
-
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2775
Kiely
Partial purification and kinet ...
Bos taurus
Biochim. Biophys. Acta
293
534-541
1973
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3
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3
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1
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2776
Domagk
Purification and properties of ...
Cyberlindnera jadinii
Eur. J. Biochem.
38
259-264
1973
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1
4
1
1
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2
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1
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1
1
1
1
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1
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1
1
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1
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4
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1
1
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1
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1
1
1
1
1
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1
1
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-
-
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-
2624
Noltmann
-
Aldose-ketose isomerases ...
Klebsiella aerogenes, Saccharomyces cerevisiae, Echinococcus granulosus, Homo sapiens, Pediococcus pentosaceus, Rhodospirillum rubrum, Spinacia oleracea
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
6
271-354
1972
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7
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3
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7
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7
1
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3
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1
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7
1
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