Cloned (Comment) | Organism |
---|---|
- |
Trypanosoma cruzi |
Protein Variants | Comment | Organism |
---|---|---|
C69A | no catalytic activity | Trypanosoma cruzi |
H102A | kinetics severeyl impaired with substrate ribose 5-phosphate, but not affected with substrate ribulose 5-phosphate | Trypanosoma cruzi |
H11A | 6fold increase in Km value, 8fold increase in kcat value, decrease in stability to freezing and thawing | Trypanosoma cruzi |
H138A | little variations in kinetics compared to wild-type | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-phospho-D-erythronate | - |
Trypanosoma cruzi | |
4-phospho-D-erythronhydrazide | - |
Trypanosoma cruzi | |
4-phospho-D-erythronohydroxamic acid | competitive | Trypanosoma cruzi | |
5-phospho-D-ribonohydroxamic acid | - |
Trypanosoma cruzi | |
iodoacetamide | 5 mM, complete inhibition | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
D-ribulose 5-diphosphate | wild-type, pH 8.4, 25°C | Trypanosoma cruzi | |
2 | - |
D-Ribose 5-diphosphate | mutant H102A, pH 8.4, 25°C | Trypanosoma cruzi | |
2 | 3 | D-Ribose 5-diphosphate | muant H11A, pH 8.4, 25°C | Trypanosoma cruzi | |
2.5 | - |
D-ribulose 5-diphosphate | mutant H102A, pH 8.4, 25°C | Trypanosoma cruzi | |
4 | - |
D-Ribose 5-diphosphate | wild-type, pH 8.4, 25°C | Trypanosoma cruzi | |
10 | - |
D-Ribose 5-diphosphate | mutant H138A, pH 8.4, 25°C | Trypanosoma cruzi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme is not metal-dependent | Trypanosoma cruzi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17400 | - |
2 * 17400, calculated, 2 * 18000, SDS-PAGE | Trypanosoma cruzi |
18000 | - |
2 * 17400, calculated, 2 * 18000, SDS-PAGE | Trypanosoma cruzi |
39600 | - |
gel filtration | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | Q4CQE2 | type B enzyme | - |
Trypanosoma cruzi CL Brener | Q4CQE2 | type B enzyme | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
amastigote | - |
Trypanosoma cruzi | - |
epimastigote | highest expression | Trypanosoma cruzi | - |
trypomastigote | metacyclic and in cell-culture | Trypanosoma cruzi | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribose 5-diphosphate | - |
Trypanosoma cruzi | D-ribulose 5-diphosphate | - |
r | |
D-ribose 5-diphosphate | - |
Trypanosoma cruzi CL Brener | D-ribulose 5-diphosphate | - |
r | |
D-ribulose 5-diphosphate | - |
Trypanosoma cruzi | D-ribose 5-diphosphate | - |
r | |
D-ribulose 5-diphosphate | - |
Trypanosoma cruzi CL Brener | D-ribose 5-diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 17400, calculated, 2 * 18000, SDS-PAGE | Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
D-Ribose 5-diphosphate | mutant H102A, pH 8.4, 25°C | Trypanosoma cruzi | |
4.7 | - |
D-ribulose 5-diphosphate | wild-type, pH 8.4, 25°C | Trypanosoma cruzi | |
10 | - |
D-Ribose 5-diphosphate | mutant H138A, pH 8.4, 25°C | Trypanosoma cruzi | |
10.7 | - |
D-ribulose 5-diphosphate | mutant H102A, pH 8.4, 25°C | Trypanosoma cruzi | |
12 | - |
D-Ribose 5-diphosphate | wild-type, pH 8.4, 25°C | Trypanosoma cruzi | |
97 | - |
D-Ribose 5-diphosphate | muant H11A, pH 8.4, 25°C | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | broad | Trypanosoma cruzi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
4-phospho-D-erythronohydroxamic acid | pH 8.4, 25°C | Trypanosoma cruzi |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
IC50 values of 5-phospho-D-ribonohydroxamic acid and 4-phospho-D-erythronhydrazide are above 10 mM | Trypanosoma cruzi | additional information | |
0.7 | - |
pH 8.4, 25°C | Trypanosoma cruzi | 4-phospho-D-erythronohydroxamic acid | |
5 | - |
pH 8.4, 25°C | Trypanosoma cruzi | 4-phospho-D-erythronate |