Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
D254R/D256R | complete loss of activity | Thermus thermophilus |
D256R | the mutant shows an increase in the specificity on D-lyxose, L-arabinose and D-mannose | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | about 58% residual activity at 1 mM | Thermus thermophilus | |
Cu2+ | less than 10% residual activity at 1 mM | Thermus thermophilus | |
Mg2+ | about 35% residual activity at 1 mM | Thermus thermophilus | |
Ni2+ | less than 5% residual activity at 1 mM | Thermus thermophilus | |
Zn2+ | complete inhibition at 1 mM | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
138 | - |
L-arabinose | mutant enzyme D256R, at pH 7.0 and 85°C | Thermus thermophilus | |
185 | - |
D-mannose | mutant enzyme D256R, at pH 7.0 and 85°C | Thermus thermophilus | |
215 | - |
D-Lyxose | mutant enzyme D256R, at pH 7.0 and 85°C | Thermus thermophilus | |
605 | - |
D-xylose | wild type enzyme, at pH 7.0 and 85°C | Thermus thermophilus | |
1005 | - |
D-mannose | wild type enzyme, at pH 7.0 and 85°C | Thermus thermophilus | |
1450 | - |
L-arabinose | wild type enzyme, at pH 7.0 and 85°C | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | maximal activity with 1 mM Mn2+ | Thermus thermophilus | |
additional information | not stimulated by K+, Ni2+ and Zn2+ | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
x * 44000, SDS-PAGE | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Xylose | Thermus thermophilus | - |
D-Xylulose | - |
r | |
D-Xylose | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
D-Xylulose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | P26997 | - |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | P26997 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Thermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
132 | - |
wild type enzyme, with L-arabinose as substrate, at pH 7.0 and 85°C | Thermus thermophilus |
273 | - |
wild type enzyme, with D-mannose as substrate, at pH 7.0 and 85°C | Thermus thermophilus |
330 | - |
mutant enzyme D256R, with L-arabinose as substrate, at pH 7.0 and 85°C | Thermus thermophilus |
380 | - |
wild type enzyme, with D-lyxose as substrate, at pH 7.0 and 85°C | Thermus thermophilus |
682 | - |
mutant enzyme D256R, with D-mannose as substrate, at pH 7.0 and 85°C | Thermus thermophilus |
950 | - |
mutant enzyme D256R, with D-lyxose as substrate, at pH 7.0 and 85°C | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose | - |
Thermus thermophilus | D-mannose | - |
r | |
D-Glucose | - |
Thermus thermophilus | D-Fructose | - |
r | |
D-Glucose | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | D-Fructose | - |
r | |
D-Lyxose | - |
Thermus thermophilus | D-Xylulose | - |
r | |
D-Lyxose | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | D-Xylulose | - |
r | |
D-Mannose | - |
Thermus thermophilus | D-Fructose | - |
r | |
D-Mannose | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | D-Fructose | - |
r | |
D-Xylose | - |
Thermus thermophilus | D-Xylulose | - |
r | |
D-Xylose | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | D-Xylulose | - |
r | |
L-arabinose | - |
Thermus thermophilus | L-ribulose | - |
r | |
L-arabinose | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | L-ribulose | - |
r | |
L-ribulose | - |
Thermus thermophilus | L-ribose | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 44000, SDS-PAGE | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
D-xylose: ketol-isomerase | - |
Thermus thermophilus |
XYLA | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Thermus thermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 95 | about 52% activity at 55°C, about 65% activity at 60°C, about 75% activity at 65°C, about 78% activity at 70°C, about 85% activity at 75°C, about 95% activity at 80°C, about 78% activity at 90°C, and about 52% activity at 95°C | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 8 | D-mannose | mutant enzyme D256R, at pH 7.0 and 85°C | Thermus thermophilus | |
6.5 | - |
L-arabinose | wild type enzyme, at pH 7.0 and 85°C | Thermus thermophilus | |
13 | - |
L-arabinose | mutant enzyme D256R, at pH 7.0 and 85°C | Thermus thermophilus | |
18 | - |
D-mannose | wild type enzyme, at pH 7.0 and 85°C | Thermus thermophilus | |
25 | - |
D-Lyxose | wild type enzyme, at pH 7.0 and 85°C | Thermus thermophilus | |
38 | - |
D-Lyxose | mutant enzyme D256R, at pH 7.0 and 85°C | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Thermus thermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | about 40% activity at pH 5.0, about 60% activity at pH 5.5, about 88% activity at pH 6.0 and 8.0, about 92% activity at pH 6.5 and 7.5, about 80% activity at pH 8.5, about 75% activity at pH 9.0 | Thermus thermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
L-arabinose | wild type enzyme, at pH 7.0 and 85°C | Thermus thermophilus | |
0.017 | - |
D-mannose | wild type enzyme, at pH 7.0 and 85°C | Thermus thermophilus | |
0.041 | - |
D-Lyxose | wild type enzyme, at pH 7.0 and 85°C | Thermus thermophilus | |
0.09 | - |
L-arabinose | mutant enzyme D256R, at pH 7.0 and 85°C | Thermus thermophilus | |
0.15 | - |
D-mannose | mutant enzyme D256R, at pH 7.0 and 85°C | Thermus thermophilus | |
0.17 | - |
D-Lyxose | mutant enzyme D256R, at pH 7.0 and 85°C | Thermus thermophilus |