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Literature summary for 5.3.1.5 extracted from

  • Umemoto, Y.; Shibata, T.; Araki, T.
    D-Xylose isomerase from a marine bacterium, Vibrio sp. strain XY-214, and D-xylulose production from beta-1,3-xylan (2012), Mar. Biotechnol., 14, 10-20.
    View publication on PubMed
Search for more literature for 5.3.1.5

Application

Application Comment Organism
synthesis the combination of TxyA, XloA, and XylA is useful tool for the D-xylulose production from beta-1,3-xylan Vibrio sp.

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Vibrio sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.93
-
 D-xylose in 50 mM sodium phosphate buffer (pH 7.5), at 40°C Vibrio sp.
187
-
 D-glucose in 50 mM sodium phosphate buffer (pH 7.5), at 40°C Vibrio sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 59% activity compared to Mn2+ Vibrio sp.
Mg2+ recombinant XylA requires the addition of Mg2+ for optimum activity (100%) Vibrio sp.
Mn2+ 41% activity compared to Mn2+ Vibrio sp.
additional information addition of Ca2+, Zn2+, Fe2+, or Cu2+ is not effective Vibrio sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 4 * 48000, SDS-PAGE Vibrio sp.
49264
-
 4 * 49264, calculated from amino acid sequence Vibrio sp.
190000
-
 gel filtration Vibrio sp.

Organism

Organism UniProt Comment Textmining
Vibrio sp. C7G532
-
-
Vibrio sp. XY-214 C7G532
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Ni-chelating column chromatography Vibrio sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Glucose
-
 Vibrio sp. D-Fructose
-
 ?
D-Glucose
-
 Vibrio sp. XY-214 D-Fructose
-
 ?
D-Xylose
-
 Vibrio sp. D-Xylulose
-
 ?
D-Xylose
-
 Vibrio sp. XY-214 D-Xylulose
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 48000, SDS-PAGE Vibrio sp.
homotetramer 4 * 49264, calculated from amino acid sequence Vibrio sp.

Synonyms

Synonyms Comment Organism
XYLA
-
 Vibrio sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Vibrio sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 65
-
 Vibrio sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 70 the enzyme is stable up to 50°C for 20 min. However, only 34% of the maximal activity is observed after preincubation at 60°C for 20 min, and no activity is observed after preincubation at 70°C for 20 min Vibrio sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.54
-
 D-glucose in 50 mM sodium phosphate buffer (pH 7.5), at 40°C Vibrio sp.
47
-
 D-xylose in 50 mM sodium phosphate buffer (pH 7.5), at 40°C Vibrio sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Vibrio sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 11
-
 Vibrio sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.024
-
 D-glucose in 50 mM sodium phosphate buffer (pH 7.5), at 40°C Vibrio sp.
5.93
-
 D-xylose in 50 mM sodium phosphate buffer (pH 7.5), at 40°C Vibrio sp.