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Literature summary for 5.3.1.5 extracted from

  • Fan, L.; Zhang, Y.; Qu, W.; Wang, J.; Shao, W.
    Cloning and analysis of the xylAB operon and characterization of xylose isomerase from Thermoanaerobacter ethanolicus (2011), Biotechnol. Lett., 33, 593-598.
    View publication on PubMed
Search for more literature for 5.3.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH10B cells Thermoanaerobacter ethanolicus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ less than 10% residual activity for D-xylose and D-glucose isomerase activities of XylA at 1 mM Zn2+ Thermoanaerobacter ethanolicus
Cu2+ complete inhibition of D-xylose and D-glucose isomerase activities of XylA at 1 mM Cu2+ Thermoanaerobacter ethanolicus
EDTA less than 10% residual activity for D-xylose and D-glucose isomerase activities of XylA at 1 mM Zn2+ Thermoanaerobacter ethanolicus
Zn2+ less than 10% residual activity for D-glucose isomerase activity and about 10% residual activity for D-xylose activity of XylA at 1 mM Zn2+ Thermoanaerobacter ethanolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11
-
 D-xylose at 80°C in 100 mM MOPS buffer, pH 7.0 Thermoanaerobacter ethanolicus

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ D-xylose and D-glucose isomerase activities of XylA are enhanced by Co2+. When 1 mM Co2+ and 10 mM Mg2+ are added together, the activity of XylA is increased by about 10% compared to 1 mM Co2+ only Thermoanaerobacter ethanolicus
Mg2+ D-xylose and D-glucose isomerase activities of XylA are enhanced by Mg2+ Thermoanaerobacter ethanolicus
Mn2+ D-xylose isomerase activity of XylA is enhanced by Mn2+ Thermoanaerobacter ethanolicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, SDS-PAGE Thermoanaerobacter ethanolicus

Organism

Organism UniProt Comment Textmining
Thermoanaerobacter ethanolicus D2DK62
-
-
Thermoanaerobacter ethanolicus JW200 (ATCC 31550) D2DK62
-
-

Purification (Commentary)

Purification (Comment) Organism
heat treatment and DEAE-Sepharose column chromatography Thermoanaerobacter ethanolicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3
-
 crude extract, at pH 7.0 and 85°C Thermoanaerobacter ethanolicus
17
-
 after 6fold purification, at pH 7.0 and 85°C Thermoanaerobacter ethanolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Xylose
-
 Thermoanaerobacter ethanolicus D-Xylulose
-
 ?
D-Xylose
-
 Thermoanaerobacter ethanolicus JW200 (ATCC 31550) D-Xylulose
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Thermoanaerobacter ethanolicus

Synonyms

Synonyms Comment Organism
XYLA
-
 Thermoanaerobacter ethanolicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
 Thermoanaerobacter ethanolicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 95
-
 Thermoanaerobacter ethanolicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
 the enzyme has a half-life of 1 h at 85°C Thermoanaerobacter ethanolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Thermoanaerobacter ethanolicus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8
-
 Thermoanaerobacter ethanolicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 10 the enzyme is stable for 1 h from pH 5.5 to 10.0 at 60°C Thermoanaerobacter ethanolicus

General Information

General Information Comment Organism
metabolism D-xylose isomerase is essential for xylose catabolism Thermoanaerobacter ethanolicus