Cloned (Comment) | Organism |
---|---|
expression of mutant enzymes in Escherichia coli strain BL21 (DE3) | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
N91D | site-directed mutagenesis | Thermus thermophilus |
N91D/D375G | site-directed mutagenesis, the mutant shows increased activity but reduced thermostability compared to the wild-type enzyme | Thermus thermophilus |
N91D/D375G/V385A | site-directed mutagenesis, the mutant shows increased activity but reduced thermostability compared to the wild-type enzyme | Thermus thermophilus |
N91D/K355A | site-directed mutagenesis, the mutant shows increased activity but reduced thermostability compared to the wild-type enzyme | Thermus thermophilus |
N91D/V144A | site-directed mutagenesis, the mutant shows increased activity but reduced thermostability compared to the wild-type enzyme | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.7 | - |
D-xylose | pH 7.0, 60°C, mutant N91D/D375G | Thermus thermophilus | |
11.8 | - |
D-xylose | pH 7.0, 60°C, mutant N91D/K355A | Thermus thermophilus | |
15.2 | - |
D-xylose | pH 7.0, 60°C, mutant N91D | Thermus thermophilus | |
21.1 | - |
D-xylose | pH 7.0, 60°C, mutant N91D/D375G/V385A | Thermus thermophilus | |
27.1 | - |
D-xylose | pH 7.0, 60°C, mutant N91D/V144A | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Xylose | Thermus thermophilus | - |
D-Xylulose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | P26997 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Xylose | - |
Thermus thermophilus | D-Xylulose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Thermus thermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 90 | temperature profiles of recombinant mutant enzymes, overview | Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 90 | thermostability of recombinant mutant enzymes, overview | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
50.7 | - |
D-xylose | pH 7.0, 60°C, mutant N91D | Thermus thermophilus | |
61.65 | - |
D-xylose | pH 7.0, 60°C, mutant N91D/D375G/V385A | Thermus thermophilus | |
68.39 | - |
D-xylose | pH 7.0, 60°C, mutant N91D/D375G | Thermus thermophilus | |
116 | - |
D-xylose | pH 7.0, 60°C, mutant N91D/K355A | Thermus thermophilus | |
160.5 | - |
D-xylose | pH 7.0, 60°C, mutant N91D/V144A | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Thermus thermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | pH profiles of recombinant mutant enzymes, overview | Thermus thermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.92 | - |
D-xylose | pH 7.0, 60°C, mutant N91D/D375G/V385A | Thermus thermophilus | |
3.35 | - |
D-xylose | pH 7.0, 60°C, mutant N91D | Thermus thermophilus | |
5.87 | - |
D-xylose | pH 7.0, 60°C, mutant N91D/D375G | Thermus thermophilus | |
5.92 | - |
D-xylose | pH 7.0, 60°C, mutant N91D/V144A | Thermus thermophilus | |
9.8 | - |
D-xylose | pH 7.0, 60°C, mutant N91D/K355A | Thermus thermophilus |