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Literature summary for 5.3.1.5 extracted from

  • Brat, D.; Boles, E.; Wiedemann, B.
    Functional expression of a bacterial xylose isomerase in Saccharomyces cerevisiae (2009), Appl. Environ. Microbiol., 75, 2304-2311.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.1.5

Cloned(Commentary)

Cloned (Comment) Organism
phylogenetic analysis and enzyme sequence comparisons, functional expression of codon-optimized enzyme in Saccharomyces cerevisiae confers on the yeast cells the ability to metabolize D-xylose and to use it as the sole carbon and energy source. The recombinant enzyme shows reduced sensitivity to inhibition by xylitol Piromyces sp.
phylogenetic analysis and enzyme sequence comparisons, functional expression of codon-optimized enzyme in Saccharomyces cerevisiae confers on the yeast cells the ability to metabolize D-xylose and to use it as the sole carbon and energy source. The recombinant enzyme shows reduced sensitivity to inhibition by xylitol Lachnoclostridium phytofermentans

Inhibitors

Inhibitors Comment Organism Structure
xylitol
-
 Lachnoclostridium phytofermentans
xylitol
-
 Piromyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
49.9
-
 D-xylose pH 7.5, recombinant enzyme Piromyces sp.
61.9
-
 D-xylose pH 7.5, native enzyme Lachnoclostridium phytofermentans
66
-
 D-xylose pH 7.5, recombinant enzyme Lachnoclostridium phytofermentans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Piromyces sp.
Mg2+ required Lachnoclostridium phytofermentans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Xylose Piromyces sp.
-
 D-Xylulose
-
 ?
D-Xylose Lachnoclostridium phytofermentans
-
 D-Xylulose
-
 ?

Organism

Organism UniProt Comment Textmining
Lachnoclostridium phytofermentans
-
-
-
Piromyces sp.
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0076
-
 native enzyme in cell extract Lachnoclostridium phytofermentans
0.0344
-
 recombinant enzyme in yeast cell extract Lachnoclostridium phytofermentans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Xylose
-
 Piromyces sp. D-Xylulose
-
 ?
D-Xylose
-
 Lachnoclostridium phytofermentans D-Xylulose
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Piromyces sp.
7.5
-
 assay at Lachnoclostridium phytofermentans