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Literature summary for 5.3.1.5 extracted from
- Engineering Pseudomonas putida S12 for efficient utilization of D-xylose and L-arabinose (2008), Appl. Environ. Microbiol., 74, 5031-5037.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | putative use of lignocellulosic biomass as feedstock for the chemical industry | Escherichia coli |
| synthesis | expression of Escherichia coli xylose isomerase and xylulokinase in Pseudomonas putida S12 for efficient utilization of D-xylose and L-arabinose. After laboratory evolution of strains by repeated transfer to fresh minimal medium with xylose, a strain that efficiently utilizes xylose at a considerably improved growth rate can be obtained. The high yield can be attributed in part to glucose dehydrogenase inactivity, whereas the improved growth rate may be connected to alterations in the primary metabolism. The evolved D-xylose-utilizing strain metabolizes L-arabinose as efficiently as D-xylose, while its ability to utilize glucose is not affected | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning and functional expression of enzymes from Escherichia coli in Pseudomonas putida | Escherichia coli |
| expression in Pseudomonas putida S12 | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | included in assay medium | Escherichia coli |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Xylose | Escherichia coli | - |
D-Xylulose | - |
? | |
| L-arabinose | Escherichia coli | - |
L-ribulose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | Pseudomonas strain S12 containing xylose isomerase gene and xylulokinase gene from Escherichia coli strain DH5 alpha | Escherichia coli | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.034 | - |
Pseudomonas putida S12 strain xylAB containing xylose isomerase and xylulokinase gene from Escherichia coli | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Xylose | - |
Escherichia coli | D-Xylulose | - |
? | |
| L-arabinose | - |
Escherichia coli | L-ribulose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| xylose isomerase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Escherichia coli |
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