Literature summary for 5.3.1.5 extracted from

van Maris, A.J.; Winkler, A.A.; Kuyper, M.; de Laat, W.T.; van Dijken, J.P.; Pronk, J.T.
Development of efficient xylose fermentation in Saccharomyces cerevisiae: xylose isomerase as a key component (2007), Adv. Biochem. Eng. Biotechnol., 108, 179-204.

Search for more literature for 5.3.1.5

Application

Application	Comment	Organism
energy production	engineering of Saccharomyces cerevisiae for alcoholic fermentation of D-xylose	Thermus thermophilus
energy production	engineering Saccharomyces cerevisiae for alcoholic fermentation of D-xylose	Piromyces sp.

Cloned(Commentary)

Cloned (Comment)	Organism
Saccharomyces cerevisiae is transformed with a gene encoding xylose isomerase (Thermus thermophilus)	Thermus thermophilus
Saccharomyces cerevisiae is transformed with a gene encoding xylose isomerase originating from Piromyces sp.	Piromyces sp.

Protein Variants

Protein Variants	Comment	Organism
additional information	Saccharomyces cerevisiae: five enzymes of non-oxidative pentose-phosphate-pathway are induced, a non-specific aldose reductase is deleted	Thermus thermophilus
additional information	Saccharomyces cerevisiae: five enzymes of non-oxidative pentose-phosphate-pathway are induced, a unspecific aldose reductase is deleted	Piromyces sp.

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	depends on the origin of the enzyme	Thermus thermophilus
Co2+	depends on the origin of the enzyme	Piromyces sp.
Mg2+	depends on the origin of the enzyme	Thermus thermophilus
Mg2+	depends on the origin of the enzyme	Piromyces sp.
Mn2+	depends on the origin of the enzyme	Thermus thermophilus
Mn2+	depends on the origin of the enzyme	Piromyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-Xylose	Thermus thermophilus	-	D-Xylulose	-	r
D-Xylose	Piromyces sp.	-	D-Xylulose	-	r

Organism

Organism	UniProt	Comment	Textmining
no activity in Saccharomyces cerevisiae	-	-	-
Piromyces sp.	-	source of xylose isomerase gene, a phylogenetic tree of the diversity of xylose isomerase genes is given - based on GenBank database	-
Thermus thermophilus	-	source of xylose isomerase gene, a phylogenetic tree of the diversity of xylose isomerase genes is given - based on GenBank database	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	measurement of the degradation of xylose and glucose	Thermus thermophilus
additional information	-	measurement of the degradation of xylose and glucose	Piromyces sp.
additional information	-	measurement of the production of ethanol, glycerol, organic acids, xylitol, CO2	Thermus thermophilus
additional information	-	measurement of the production of ethanol, glycerol, organic acids, xylitol, CO2	Piromyces sp.
additional information	-	strain is cultivated under aerobic and anaerobic conditions	Thermus thermophilus
additional information	-	strains are cultivated under aerobic and anaerobic conditions	Piromyces sp.
0.017	-	Saccharomyces cerevisiae overexprssing five own enzymes of pentose phosphate pathway, deletion of an unspecific aldose reductase, overexpressing xylose isomerase from Piromyces sp., 30°C	Piromyces sp.
0.04	-	Saccharomyces cerevisiae overexpressing xylose isomerase from Thermus thermophilus, 30°C	Thermus thermophilus
1	-	Saccharomyces cerevisiae overexpressing xylose isomerase from Thermus thermophilus, 85°C	Thermus thermophilus
1.1	-	Saccharomyces cerevisiae overexprssing xylose isomerase from Piromyces sp., 30°C	Piromyces sp.
1.1	-	Saccharomyces cerevisiae strain CEN.PK overexprssing xylose isomerase (xylA gene) from Piromyces sp., TPI1 promoter, 30°C	Piromyces sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Glucose	-	Thermus thermophilus	D-Fructose	-	r
D-Glucose	-	Piromyces sp.	D-Fructose	-	r
D-Xylose	-	Thermus thermophilus	D-Xylulose	-	r
D-Xylose	-	Piromyces sp.	D-Xylulose	-	r

Synonyms

Synonyms	Comment	Organism
D-xylose ketol isomerase	-	Thermus thermophilus
D-xylose ketol isomerase	-	Piromyces sp.
xylose isomerase	-	Thermus thermophilus
xylose isomerase	-	no activity in Saccharomyces cerevisiae
xylose isomerase	-	Piromyces sp.

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
60	80	-	Thermus thermophilus
60	80	-	Piromyces sp.

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Release 2023.1 (January 2023)