Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Lactococcus lactis |
Protein Variants | Comment | Organism |
---|---|---|
E407K | 2% of strain IO-1 wild-type activity | Lactococcus lactis |
K407E | 92% of wild-type activity | Lactococcus lactis |
R202M | 9% of strain IO-1 wild-type activity | Lactococcus lactis |
R202M/V275A | 26% of strain IO-1 wild-type activity | Lactococcus lactis |
R202M/Y218D | 9% of strain IO-1 wild-type activity | Lactococcus lactis |
R202M/Y218D/V275A | 62% of strain IO-1 wild-type activity and soluble | Lactococcus lactis |
S247A | 97% of wild-type activity | Lactococcus lactis |
S247A/K407E | 79% of wild-type activity | Lactococcus lactis |
S247A/S388T | 27% of wild-type activity | Lactococcus lactis |
S388T | insoluble and 8% of wild-type activity | Lactococcus lactis |
S388T/K407E | soluble and 50% of wild-type activity | Lactococcus lactis |
T388S | 11% of strain IO-1 wild-type activity | Lactococcus lactis |
Y218D | 14% of strain IO-1 wild-type activity | Lactococcus lactis |
Y218D/V275A | 24% of strain IO-1 wild-type activity | Lactococcus lactis |
Y275A | 24% of strain IO-1 wild-type activity | Lactococcus lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.25 | - |
D-xylose | wild-type | Lactococcus lactis | |
3.46 | - |
D-xylose | mutant R202M/Y218D/V275A | Lactococcus lactis | |
4.4 | - |
D-xylose | mutant S388T/K407E | Lactococcus lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
SDS-PAGE | Lactococcus lactis |
49700 | - |
calculated from amino acid sequence | Lactococcus lactis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Xylose | Lactococcus lactis | - |
D-Xylulose | - |
? | |
D-Xylose | Lactococcus lactis IO-1 (Xyl+) | - |
D-Xylulose | - |
? | |
D-Xylose | Lactococcus lactis 210 (Xyl-) | - |
D-Xylulose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | - |
- |
- |
Lactococcus lactis 210 (Xyl-) | - |
- |
- |
Lactococcus lactis IO-1 (Xyl+) | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified enzyme is insoluble and inactive | Lactococcus lactis |
purified enzyme is soluble | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Xylose | - |
Lactococcus lactis | D-Xylulose | - |
? | |
D-Xylose | - |
Lactococcus lactis IO-1 (Xyl+) | D-Xylulose | - |
? | |
D-Xylose | - |
Lactococcus lactis 210 (Xyl-) | D-Xylulose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
XI | - |
Lactococcus lactis |
xylose isomerase | - |
Lactococcus lactis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Lactococcus lactis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
74 | - |
activity decreases dramatically above 74°C | Lactococcus lactis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
124 | - |
D-xylose | mutant S388T/K407E | Lactococcus lactis | |
142 | - |
D-xylose | mutant R202M/Y218D/V275A | Lactococcus lactis | |
184 | - |
D-xylose | wild-type | Lactococcus lactis |